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Smc5/6 coordinates formation and resolution of joint molecules with chromosome morphology to ensure meiotic divisions
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Tang, Shangming, Copsey, Alice, Jordan, Philip W., Blitzblau, Hannah G., Newcombe, Sonya, Chan, Andrew Chi-ho, Newnham, Louise, Li, Zhaobo, Gray, Stephen, Herbert, Alex D., Arumugam, Prakash , Hochwagen, Andreas, Hunter, Neil and Hoffmann, Eva (2013) Smc5/6 coordinates formation and resolution of joint molecules with chromosome morphology to ensure meiotic divisions. PLoS Genetics, Volume 9 (Number 12). Article number e1004071. doi:10.1371/journal.pgen.1004071
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WRAP_Arumugam_journal.pgen.1004071.pdf - Published Version Available under License Creative Commons Attribution. Download (7Mb) | Preview |
Official URL: http://dx.doi.org/10.1371/journal.pgen.1004071
Abstract
During meiosis, Structural Maintenance of Chromosome (SMC) complexes underpin two fundamental features of meiosis: homologous recombination and chromosome segregation. While meiotic functions of the cohesin and condensin complexes have been delineated, the role of the third SMC complex, Smc5/6, remains enigmatic. Here we identify specific, essential meiotic functions for the Smc5/6 complex in homologous recombination and the regulation of cohesin. We show that Smc5/6 is enriched at centromeres and cohesin-association sites where it regulates sister-chromatid cohesion and the timely removal of cohesin from chromosomal arms, respectively. Smc5/6 also localizes to recombination hotspots, where it promotes normal formation and resolution of a subset of joint-molecule intermediates. In this regard, Smc5/6 functions independently of the major crossover pathway defined by the MutLγ complex. Furthermore, we show that Smc5/6 is required for stable chromosomal localization of the XPF-family endonuclease, Mus81-Mms4Eme1. Our data suggest that the Smc5/6 complex is required for specific recombination and chromosomal processes throughout meiosis and that in its absence, attempts at cell division with unresolved joint molecules and residual cohesin lead to severe recombination-induced meiotic catastrophe
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Subjects: | Q Science > QH Natural history > QH301 Biology | ||||
| Divisions: | Faculty of Science > Life Sciences (2010- ) | ||||
| Library of Congress Subject Headings (LCSH): | Meiosis, Chromosomes, Morphology, Molecules | ||||
| Journal or Publication Title: | PLoS Genetics | ||||
| Publisher: | Public Library of Science | ||||
| ISSN: | 1553-7390 | ||||
| Official Date: | December 2013 | ||||
| Dates: |
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| Volume: | Volume 9 | ||||
| Number: | Number 12 | ||||
| Page Range: | Article number e1004071 | ||||
| DOI: | 10.1371/journal.pgen.1004071 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Open Access | ||||
| Funder: | National Institute of Environmental Health Sciences (NIEHS), Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Charles A. King Trust Postdoctoral Research Fellowship Program, Medical Research Council (Great Britain) (MRC), National Institutes of Health (U.S.) (NIH), National Institute of General Medical Sciences (U.S.) (NIGMS) | ||||
| Grant number: | T32 ES007058-33 (NIEHS), BB/G00353X/1 (BBSRC), GM084955 (NIH/NIGMS), R01 GM088248 (NIH) |
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