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Absorption mode FTICR mass spectrometry imaging
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Smith, Donald Francis, Kilgour, David P. A., Konijnenburg, Marco, O’Connor, Peter B. and Heeren, Ron M. A. (2013) Absorption mode FTICR mass spectrometry imaging. Analytical Chemistry, Volume 85 (Number 23). pp. 11180-11184. doi:10.1021/ac403039t ISSN 0003-2700.
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Official URL: http://dx.doi.org/10.1021/ac403039t
Abstract
Fourier transform ion cyclotron resonance mass spectrometry offers the highest mass resolving power for molecular imaging experiments. This high mass resolving power ensures that closely spaced peaks at the same nominal mass are resolved for proper image generation. Typically higher magnetic fields are used to increase mass resolving power. However, a gain in mass resolving power can also be realized by phase correction of the data for absorption mode display. In addition to mass resolving power, absorption mode offers higher mass accuracy and signal-to-noise ratio over the conventional magnitude mode. Here, we present the first use of absorption mode for Fourier transform ion cyclotron resonance mass spectrometry imaging. The Autophaser algorithm is used to phase correct each spectrum (pixel) in the image, and then, these parameters are used by the Chameleon work-flow based data processing software to generate absorption mode “Datacubes” for image and spectral viewing. Absorption mode reveals new mass and spatial features that are not resolved in magnitude mode and results in improved selected ion image contrast.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QD Chemistry | ||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||
Library of Congress Subject Headings (LCSH): | Ion cyclotron resonance spectrometry, Fourier transform spectroscopy, Mass spectrometry, Absorption spectra | ||||
Journal or Publication Title: | Analytical Chemistry | ||||
Publisher: | American Chemical Society | ||||
ISSN: | 0003-2700 | ||||
Official Date: | 2013 | ||||
Dates: |
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Volume: | Volume 85 | ||||
Number: | Number 23 | ||||
Page Range: | pp. 11180-11184 | ||||
DOI: | 10.1021/ac403039t | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access |
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