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The host-range tdCE phenotype of Chandipura virus is determined by mutations in the polymerase gene
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Stock, Emily, Marriott, Anthony C. and Easton, A. J. (Andrew J.) (2014) The host-range tdCE phenotype of Chandipura virus is determined by mutations in the polymerase gene. Journal of General Virology, Volume 95 (Number 1). pp. 38-43. doi:10.1099/vir.0.059204-0
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WRAP_Easton_Stock et al accepted.pdf - Accepted Version Download (669Kb) | Preview |
Official URL: http://dx.doi.org/10.1099/vir.0.059204-0
Abstract
The emerging arbovirus Chandipura virus (CV) has been implicated in epidemics of acute encephalitis in India with high mortality rates. The isolation of temperature-dependent host-range (tdCE) mutants, which are impaired in growth at 39 °C in chick embryo (CE) cells but not in monkey cells, highlights a dependence on undetermined host factors. We have characterized three tdCE mutants, each containing one or more coding mutations in the RNA polymerase gene and two containing additional mutations in the attachment protein gene. Using reverse genetics, we showed that a single amino acid change in the virus polymerase of each mutant was responsible for the host-range specificity. In CE cells at the non-permissive temperature, the discrete cytoplasmic replication complexes seen in mammalian cells or at the permissive temperature in CE cells were absent with the tdCE mutants, consistent with the tdCE lesions causing disruption of the replication complexes in a host-dependent manner.
| Item Type: | Journal Article | ||||
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| Subjects: | Q Science > QH Natural history > QH426 Genetics Q Science > QR Microbiology > QR355 Virology |
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
| Library of Congress Subject Headings (LCSH): | Arboviruses -- Research, DNA polymerases, Mutation (Biology), Phenotype, Host-virus relationships | ||||
| Journal or Publication Title: | Journal of General Virology | ||||
| Publisher: | Society for General Microbiology | ||||
| ISSN: | 0022-1317 | ||||
| Official Date: | October 2014 | ||||
| Dates: |
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| Volume: | Volume 95 | ||||
| Number: | Number 1 | ||||
| Page Range: | pp. 38-43 | ||||
| DOI: | 10.1099/vir.0.059204-0 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access |
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