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Manuka honey inhibits adhesion and invasion of medically important wound bacteria in vitro
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Maddocks, Sarah Elizabeth, Jenkins, Rowena Eleri, Rowlands, Richard Samuel, Purdy, Kevin J. and Cooper, Rose (2013) Manuka honey inhibits adhesion and invasion of medically important wound bacteria in vitro. Future Microbiology, Volume 8 (Number 12). pp. 1523-1536. doi:10.2217/fmb.13.126 ISSN 1746-0913.
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Official URL: http://dx.doi.org/10.2217/fmb.13.126
Abstract
Aim: To characterize the effect of manuka honey on medically important
wound bacteria in vitro, focusing on its antiadhesive properties.
Materials & methods: Crystal violet biofilm assays, fluorescent
microscopy, protein adhesion assay and gentamicin protection assay were
used to determine the impact of manuka honey on biofilm formation, human
protein binding and adherence to/invasion into human keratinocytes.
Results: Manuka honey effectively disrupted and caused extensive cell
death in biofilms of Staphylococcus aureus, Pseudomonas aeruginosa and
Streptococcus pyogenes. Sublethal doses of manuka honey inhibited
bacterial adhesion to the fibronectin, fibrinogen and collagen. Manuka
honey impaired adhesion of laboratory and clinical isolates of S.
aureus, P. aeruginosa and S. pyogenes to human keratinocytes in vitro,
and inhibited invasion by S. pyogenes and homogeneous vancomycin
intermediate S. aureus. Conclusion: Manuka honey can directly affect
bacterial cells embedded in a biofilm and exhibits antiadhesive
properties against three common wound pathogens.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QR Microbiology S Agriculture > SF Animal culture |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
Library of Congress Subject Headings (LCSH): | Honey -- Therapeutic use, Bacteria -- Adhesion, Biofilms, Staphylococcus aureus, Fibronectins | ||||
Journal or Publication Title: | Future Microbiology | ||||
Publisher: | Future Medicine Ltd. | ||||
ISSN: | 1746-0913 | ||||
Official Date: | 2013 | ||||
Dates: |
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Volume: | Volume 8 | ||||
Number: | Number 12 | ||||
Page Range: | pp. 1523-1536 | ||||
DOI: | 10.2217/fmb.13.126 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access |
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