Thomas, M. G., Covington, James A. and Wall, Mark J. (2013) A chamber for the perfusion of in vitro tissue with multiple solutions. Journal of Neurophysiology, Volume 110 (Number 1). pp. 269-277. doi:10.1152/jn.01039.2012 ISSN 0022-3077.
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Abstract
There are currently no practical systems that allow extended regions (>5 mm2) of a tissue slice in vitro to be exposed, in isolation, to changes in ionic conditions or to pharmacological manipulation. Previous work has only achieved this at the expense of access to the tissue for recording electrodes. Here, we present a chamber that allows a tissue slice to be maintained in multiple solutions, at physiological temperatures, and preserves the ability to record from the slice. We demonstrate the effectiveness of the tissue bath with respect to minimizing the mixing of the solutions, maintaining the viability of the tissue, and preserving the ability to record from the slice simultaneously
| Item Type: | Journal Article |
|---|---|
| Divisions: | Faculty of Science, Engineering and Medicine > Engineering > Engineering Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) Faculty of Science, Engineering and Medicine > Research Centres > Molecular Organisation and Assembly in Cells (MOAC) |
| Journal or Publication Title: | Journal of Neurophysiology |
| Publisher: | American Physiological Society |
| ISSN: | 0022-3077 |
| Official Date: | 1 July 2013 |
| Dates: | Date Event 1 July 2013 Published |
| Volume: | Volume 110 |
| Number: | Number 1 |
| Page Range: | pp. 269-277 |
| DOI: | 10.1152/jn.01039.2012 |
| Status: | Peer Reviewed |
| Publication Status: | Published |
| Access rights to Published version: | Restricted or Subscription Access |
| URI: | https://wrap.warwick.ac.uk/59254/ |
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