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Molecular cloning and transcriptional activity of a new Petunia calreticulin gene involved in pistil transmitting tract maturation, progamic phase, and double fertilization
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Lenartowski, Robert, Suwińska, Anna, Prusińska, Justyna, Gumowski, Krzysztof and Lenartowska, Marta (2014) Molecular cloning and transcriptional activity of a new Petunia calreticulin gene involved in pistil transmitting tract maturation, progamic phase, and double fertilization. Planta, Volume 239 (Number 2). pp. 437-454. doi:10.1007/s00425-013-1971-4
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WRAP_Prusińska_ art%3A10.1007%2Fs00425-013-1971-4.pdf - Published Version Available under License Creative Commons Attribution. Download (1527Kb) | Preview |
Official URL: http://dx.doi.org/10.1007/s00425-013-1971-4
Abstract
Calreticulin (CRT) is a highly conserved and ubiquitously expressed Ca2+-binding protein in multicellular eukaryotes. As an endoplasmic reticulum-resident protein, CRT plays a key role in many cellular processes including Ca2+ storage and release, protein synthesis, and molecular chaperoning in both animals and plants. CRT has long been suggested to play a role in plant sexual reproduction. To begin to address this possibility, we cloned and characterized the full-length cDNA of a new CRT gene (PhCRT) from Petunia. The deduced amino acid sequence of PhCRT shares homology with other known plant CRTs, and phylogenetic analysis indicates that the PhCRT cDNA clone belongs to the CRT1/CRT2 subclass. Northern blot analysis and fluorescent in situ hybridization were used to assess PhCRT gene expression in different parts of the pistil before pollination, during subsequent stages of the progamic phase, and at fertilization. The highest level of PhCRT mRNA was detected in the stigma–style part of the unpollinated pistil 1 day before anthesis and during the early stage of the progamic phase, when pollen is germinated and tubes outgrow on the stigma. In the ovary, PhCRT mRNA was most abundant after pollination and reached maximum at the late stage of the progamic phase, when pollen tubes grow into the ovules and fertilization occurs. PhCRT mRNA transcripts were seen to accumulate predominantly in transmitting tract cells of maturing and receptive stigma, in germinated pollen/growing tubes, and at the micropylar region of the ovule, where the female gametophyte is located. From these results, we suggest that PhCRT gene expression is up-regulated during secretory activity of the pistil transmitting tract cells, pollen germination and outgrowth of the tubes, and then during gamete fusion and early embryogenesis.
| Item Type: | Journal Article | ||||
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| Subjects: | Q Science > QH Natural history > QH301 Biology Q Science > QH Natural history > QH426 Genetics |
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| Divisions: | Faculty of Science > Life Sciences (2010- ) | ||||
| Library of Congress Subject Headings (LCSH): | Molecular cloning , Petunias , Calreticulin , Plant genetics | ||||
| Journal or Publication Title: | Planta | ||||
| Publisher: | Springer | ||||
| ISSN: | 0032-0935 | ||||
| Official Date: | 12 November 2014 | ||||
| Dates: |
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| Date of first compliant deposit: | 26 December 2015 | ||||
| Volume: | Volume 239 | ||||
| Number: | Number 2 | ||||
| Page Range: | pp. 437-454 | ||||
| DOI: | 10.1007/s00425-013-1971-4 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Open Access | ||||
| Funder: | Poland. Ministerstwo Nauki i Szkolnictwa Wyższego [Ministry of Science and Higher Education] (MNiSW), Uniwersytet Mikołaja Kopernika w Toruniu [Nicolaus Copernicus University in Torún] (Torun, Poland) | ||||
| Grant number: | N303 023 32/1034 (MNiSW) |
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