Ahmad, Mark, Taylor, C. R. (Charles R.), Pink, David, Burton, Kerry S., Eastwood, Daniel C., Bending, G. D. (Gary D.) and Bugg, Tim. (2010) Development of novel assays for lignin degradation : comparative analysis of bacterial and fungal lignin degraders. Molecular BioSystems, Vol.6 (No.5). pp. 815-821. ISSN 1742-206X

Full text not available from this repository.

Abstract

Two spectrophotometric assays have been developed to monitor breakdown of the lignin component of plant lignocellulose: a continuous fluorescent assay involving fluorescently modified lignin, and a UV-vis assay involving chemically nitrated lignin. These assays have been used to analyse lignin degradation activity in bacterial and fungal lignin degraders, and to identify additional soil bacteria that show activity for lignin degradation. Two soil bacteria known to act as aromatic degraders, Pseudomonas putida and Rhodococcus sp. RHA1, consistently showed activity in these assays, and these strains were shown in a small scale experiment to breakdown lignocellulose, producing a number of monocyclic phenolic products. Using milled wood lignin prepared from wheat straw, pine, and miscanthus, some bacterial lignin degraders were found to show specificity for lignin type. These assays could be used to identify novel lignin degraders for breakdown of plant lignocellulose.

Item Type:	Journal Article
Subjects:	Q Science > QD Chemistry Q Science > QP Physiology Q Science > QR Microbiology
Divisions:	Other > Institute of Advanced Study Faculty of Science > Chemistry Faculty of Science > Life Sciences (2010- ) > Warwick HRI (2004-2010)
Library of Congress Subject Headings (LCSH):	Lignin -- Biodegradation, Lignocellulose, Spectrophotometry
Journal or Publication Title:	Molecular BioSystems
Publisher:	Royal Society of Chemistry
ISSN:	1742-206X
Date:	May 2010
Volume:	Vol.6
Number:	No.5
Number of Pages:	7
Page Range:	pp. 815-821
Identification Number:	10.1039/b908966g
Status:	Peer Reviewed
Publication Status:	Published
Funder:	University of Warwick. Innovative Materials Research Centre
References:	1 E. Sjostrom, Wood Chemistry Fundamentals and Applications, Academic Press inc., London, 2nd edn, 1993, ch. 4, pp. 73–83. 2 O. Faix, Holzforschung, 1991, 45(21), 21–27. 3 S. Kelley and W. Glasser, Encyclopedia of Polymer Science and Engineering, John Wiley and sons, New York, 2nd edn, 1987, vol. 8, pp. 795–796. 4 G. Festel, Chem. Eng. Technol., 2008, 31, 715–720. 5 E. Sjo¨ stro¨ m, Wood Chemistry Fundamentals and Applications, Academic Press inc., London, 2nd edn, 1993, ch. 5, p. 118. 6 M. H. Gold and M. Alic, Microbiol. Rev., 1993, 57, 605–622. 7 T. Higuchi, Proc. Jpn. Acad., Ser. B, 2004, 80, 204–214. 8 M. Ramachandra, D. Crawford and G. Hertel, Appl. Environ. Microbiol., 1988, 54, 3057–3063. 9 D. Crawford, A. Pometto III and R. Crawford, Appl. Environ. Microbiol., 1983, 45, 898–904. 10 W. Zimmermann, J. Biotechnol., 1990, 13, 119–130. 11 E. Masai, Y. Katayama and M. Fukuda, Biosci., Biotechnol., Biochem., 2007, 71, 1–15. 12 H. Kern and T. Kirk, Appl. Environ. Microbiol., 1987, 53, 2242–2246. 13 T. Kirk, Annu. Rev. Phytopathol., 1971, 9, 185–210. 14 D. R. Herbold and L. Glaser, J. Biol. Chem., 1975, 250, 1676. 15 D. Crawford and R. Crawford, Enzyme Microb. Technol., 1980, 2, 11–22. 16 T. Higuchi, Proc. Jpn. Acad., Ser. B, 2004, 80, 204–214. 17 M. Gold, J. Glenn and M. Alic, Methods Enzymol., 1988, 161, 74–78. 18 W. Zimmerman, A. Paterson and P. Broda, Methods Enzymol., 1988, 161, 191–199. 19 G. Wells, A. Seaton and M. Stevens, J. Med. Chem., 2000, 43, 1550–1562. 20 K. Haider, J. Trojanowki and V. Sundman, Arch. Microbiol., 1978, 119, 103–106. 21 P. Williams and K. Murray, J. Bacteriol., 1974, 120, 416–423. 22 M. Seto, K. Kimbara, M. Shimura, T. Hatta, M. Fukuda and K. Yano, Appl. Environ. Microbiol., 1995, 61, 3353–3358. 23 G. V. B. Reddy, M. Sridhar and M. H. Gold, Eur. J. Biochem., 2003, 270, 284–292. 24 M. McLeod, R. Warren, W. Hsiao, N. Araki, M. Myhre, C. Femandes, D. Miyazawa, W. Wong, A. Lillquist, D. Wang, M. Dosanjh, H. Hara, A. Petrescu, R. Morin, G. Yang, J. Stott, J. Schein, H. Shin, D. Smailus, A. Siddiqui, M. Marra, S. Jones, R. Holt, F. Brinkman, K. Miyauchi, M. Fukuda, J. Davies, W. Mohn and L. Eltis, Proc. Natl. Acad. Sci. U. S. A., 2006, 103, 15582–15587. 25 Ectomycorrhizal Fungi: Key Genera in Profile, ed. J. W. G. Cairney and S. M. Chambers, Springer-Verlag, Berlin, 1999. 26 G. D. Bending and D. J. Read, Mycol. Res., 1997, 101, 1348–1354.
URI:	http://wrap.warwick.ac.uk/id/eprint/5937

Data sourced from Thomson Reuters' Web of Knowledge

Request changes to a record

Actions (login required)

View Item