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Development of novel assays for lignin degradation : comparative analysis of bacterial and fungal lignin degraders

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Ahmad, Mark, Taylor, C. R. (Charles R.), Pink, David, Burton, Kerry S., Eastwood, Daniel C., Bending, G. D. (Gary D.) and Bugg, Tim. (2010) Development of novel assays for lignin degradation : comparative analysis of bacterial and fungal lignin degraders. Molecular BioSystems, Vol.6 (No.5). pp. 815-821. ISSN 1742-206X

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Abstract

Two spectrophotometric assays have been developed to monitor breakdown of the lignin component of plant lignocellulose: a continuous fluorescent assay involving fluorescently modified lignin, and a UV-vis assay involving chemically nitrated lignin. These assays have been used to analyse lignin degradation activity in bacterial and fungal lignin degraders, and to identify additional soil bacteria that show activity for lignin degradation. Two soil bacteria known to act as aromatic degraders, Pseudomonas putida and Rhodococcus sp. RHA1, consistently showed activity in these assays, and these strains were shown in a small scale experiment to breakdown lignocellulose, producing a number of monocyclic phenolic products. Using milled wood lignin prepared from wheat straw, pine, and miscanthus, some bacterial lignin degraders were found to show specificity for lignin type. These assays could be used to identify novel lignin degraders for breakdown of plant lignocellulose.

Item Type: Journal Article
Subjects: Q Science > QD Chemistry
Q Science > QP Physiology
Q Science > QR Microbiology
Divisions: Other > Institute of Advanced Study
Faculty of Science > Chemistry
Faculty of Science > Life Sciences (2010- ) > Warwick HRI (2004-2010)
Library of Congress Subject Headings (LCSH): Lignin -- Biodegradation, Lignocellulose, Spectrophotometry
Journal or Publication Title: Molecular BioSystems
Publisher: Royal Society of Chemistry
ISSN: 1742-206X
Official Date: May 2010
Volume: Vol.6
Number: No.5
Number of Pages: 7
Page Range: pp. 815-821
Identification Number: 10.1039/b908966g
Status: Peer Reviewed
Publication Status: Published
Funder: University of Warwick. Innovative Materials Research Centre
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URI: http://wrap.warwick.ac.uk/id/eprint/5937

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