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Uterine selection of human embryos at implantation
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(2014) Uterine selection of human embryos at implantation. Scientific Reports, 4 . 3894. doi:10.1038/srep03894 ISSN 2045-2322.
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WRAP_Brosens_srep03894.pdf - Published Version Available under License Creative Commons Attribution Non-commercial Share Alike. Download (2637Kb) | Preview |
Official URL: http://dx.doi.org/10.1038/srep03894
Abstract
Human embryos frequently harbor large-scale complex chromosomal errors that impede normal development. Affected embryos may fail to implant although many first breach the endometrial epithelium and embed in the decidualizing stroma before being rejected via mechanisms that are poorly understood. Here we show that developmentally impaired human embryos elicit an endoplasmic stress response in human decidual cells. A stress response was also evident upon in vivo exposure of mouse uteri to culture medium conditioned by low-quality human embryos. By contrast, signals emanating from developmentally competent embryos activated a focused gene network enriched in metabolic enzymes and implantation factors. We further show that trypsin, a serine protease released by pre-implantation embryos, elicits Ca2+ signaling in endometrial epithelial cells. Competent human embryos triggered short-lived oscillatory Ca2+ fluxes whereas low-quality embryos caused a heightened and prolonged Ca2+ response. Thus, distinct positive and negative mechanisms contribute to active selection of human embryos at implantation.
| Item Type: | Journal Article | ||||
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| Subjects: | Q Science > QH Natural history > QH301 Biology Q Science > QH Natural history > QH426 Genetics |
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| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Translational & Experimental Medicine > Reproductive Health ( - until July 2016) Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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| Library of Congress Subject Headings (LCSH): | Embryology, Human -- Great Britain, Human embryo -- Transplantation | ||||
| Journal or Publication Title: | Scientific Reports | ||||
| Publisher: | Nature Publishing Group | ||||
| ISSN: | 2045-2322 | ||||
| Official Date: | 6 February 2014 | ||||
| Dates: |
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| Volume: | 4 | ||||
| Number of Pages: | 8 | ||||
| Article Number: | 3894 | ||||
| DOI: | 10.1038/srep03894 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Open Access (Creative Commons) | ||||
| Date of first compliant deposit: | 26 December 2015 | ||||
| Date of first compliant Open Access: | 26 December 2015 | ||||
| Funder: | Nederlandse Organisatie voor Wetenschappelijk Onderzoek [Netherlands Organisation for Scientific Research] (NWO), University Hospitals Coventry and Warwickshire NHS Trust, Genesis Research Trust |
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