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Profiling antibodies to class II HLA in transplant patient sera
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McMurtrey, Curtis, Lowe, David Philip, Buchli, Rico, Daga, Sunil, Royer, Derek, Humphrey, Alisha, Cate, Steven, Osborn, Sean, Mojsilovic, Aleksandar, VanGundy, Rodney, Bardet, Wilfried, Duty, Andrew, Mojsilovic, Danijela, Jackson, Kenneth, Stastny, Peter, Briggs, David, Zehnder, Daniel, Higgins, Rob and Hildebrand, William (2014) Profiling antibodies to class II HLA in transplant patient sera. Human Immunology, Volume 75 (Number 3). pp. 261-270. doi:10.1016/j.humimm.2013.11.015 ISSN 0198-8859.
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Official URL: http://dx.doi.org/10.1016/j.humimm.2013.11.015
Abstract
Immunizing events including pregnancy, transfusions, and transplantation promote strong alloantibody responses to HLA. Such alloantibodies to HLA preclude organ transplantation, foster hyperacute rejection, and contribute to chronic transplant failure. Diagnostic antibody-screening assays detect alloreactive antibodies, yet key attributes including antibody concentration and isotype remain largely unexplored. The goal here was to provide a detailed profile of allogeneic antibodies to class II HLA. Methodologically, alloantibodies were purified from sensitized patient sera using an HLA-DR11 immunoaffinity column and subsequently categorized. Antibodies to DR11 were found to fix complement, exist at a median serum concentration of 2.3 μg/mL, consist of all isotypes, and isotypes IgG2, IgM, and IgE were elevated. Because multimeric isotypes can confound diagnostic determinations of antibody concentration, IgM and IgA isotypes were removed and DR11-IgG tested alone. Despite removal of multimeric isotypes, patient-to-patient antibody concentrations did not correlate with MFI values. In conclusion, allogeneic antibody responses to DR11 are comprised of all antibody isotypes at differing proportions, these combined isotypes fix complement at nominal serum concentrations, and enhancements other than the removal of IgM and IgA multimeric isotypes may be required if MFI is to be used as a means of determining anti-HLA serum antibody concentrations in diagnostic clinical assays.
Item Type: | Journal Article | ||||||||||
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Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Translational & Experimental Medicine > Metabolic and Vascular Health (- until July 2016) Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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Journal or Publication Title: | Human Immunology | ||||||||||
Publisher: | Elsevier Science BV | ||||||||||
ISSN: | 0198-8859 | ||||||||||
Official Date: | March 2014 | ||||||||||
Dates: |
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Volume: | Volume 75 | ||||||||||
Number: | Number 3 | ||||||||||
Page Range: | pp. 261-270 | ||||||||||
DOI: | 10.1016/j.humimm.2013.11.015 | ||||||||||
Status: | Peer Reviewed | ||||||||||
Publication Status: | Published | ||||||||||
Access rights to Published version: | Restricted or Subscription Access |
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