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Single-cell states in the estrogen response of breast cancer cell lines
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Casale, Francesco Paolo, Giurato, Giorgio, Nassa, Giovanni, Armond, Jonathan W., Oates, Chris J., Corá, Davide, Gamba, Andrea, Mukherjee, Sach, Weisz, Alessandro and Nicodemi, Mario (2014) Single-cell states in the estrogen response of breast cancer cell lines. PLoS ONE , 9 (2). Article number e88485. doi:10.1371/journal.pone.0088485
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Official URL: http://dx.doi.org/10.1371/journal.pone.0088485
Abstract
Estrogen responsive breast cancer cell lines have been extensively studied to characterize transcriptional patterns in hormone-responsive tumors. Nevertheless, due to current technological limitations, genome-wide studies have typically been limited to population averaged data. Here we obtain, for the first time, a characterization at the single-cell level of the states and expression signatures of a hormone-starved MCF-7 cell system responding to estrogen. To do so, we employ a recently proposed model that allows for dissecting single-cell states from time-course microarray data. We show that within 32 hours following stimulation, MCF-7 cells traverse, most likely, six states, with a faster early response followed by a progressive deceleration. We also derive the genome-wide transcriptional profiles of such single-cell states and their functional characterization. Our results support a scenario where estrogen promotes cell cycle progression by controlling multiple, sequential regulatory steps, whose single-cell events are here identified.
| Item Type: | Journal Article | ||||||||
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| Subjects: | R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer) | ||||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Research Centres > Centre for Complexity Science Faculty of Science, Engineering and Medicine > Science > Statistics |
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| Library of Congress Subject Headings (LCSH): | Breast -- Cancer -- Hormone therapy, Estrogen | ||||||||
| Journal or Publication Title: | PLoS ONE | ||||||||
| Publisher: | Public Library of Science | ||||||||
| ISSN: | 1932-6203 | ||||||||
| Official Date: | 25 February 2014 | ||||||||
| Dates: |
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| Volume: | 9 | ||||||||
| Number: | 2 | ||||||||
| Number of Pages: | 11 | ||||||||
| Article Number: | Article number e88485 | ||||||||
| DOI: | 10.1371/journal.pone.0088485 | ||||||||
| Status: | Peer Reviewed | ||||||||
| Publication Status: | Published | ||||||||
| Access rights to Published version: | Open Access | ||||||||
| Funder: | Associazione italiana per la ricerca sul cancro (AIRC), Italy. Ministero dell'istruzione, dell'università e della ricerca (MIUR) | ||||||||
| Grant number: | IG-13176 (AIRC), PRIN 2010LC747T 002 (Italy) |
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