The Library
A rapid method for the determination of artemisinin and its biosynthetic precursors in Artemisia annua L. crude extracts
Tools
Suberu, John O., Song, Lijiang, Slade, Susan E., Sullivan, Neil F., Barker, Guy C. and Lapkin, Alexei (2013) A rapid method for the determination of artemisinin and its biosynthetic precursors in Artemisia annua L. crude extracts. Journal of Pharmaceutical and Biomedical Analysis, Volume 84 . pp. 269-277. doi:10.1016/j.jpba.2013.06.025 ISSN 0731-7085.
Research output not available from this repository.
Request-a-Copy directly from author or use local Library Get it For Me service.
Official URL: http://dx.doi.org/10.1016/j.jpba.2013.06.025
Abstract
A rapid high-pressure liquid chromatography (HPLC) tandem mass spectrometry (TQD) method for the determination of artemisinin, 9-epi-artemisinin, artemisitene, dihydroartemisinic acid, artemisinic acid and arteannuin B in Artemisia annua extracts is described. Detection and quantification of 9-epi-artemisinin in crude extracts are reported for the first time. In this method all six metabolites are resolved and eluted within 6 min with minimal sample preparation. A recovery of between 96.25% and 103.59% was obtained for all metabolites analysed and the standard curves were linear (r2 > 0.99) over the concentration range of 0.15–10 μg mL−1 for artemisinin, 9-epi-artemisinin, artemisitene and arteannuin B, and the range of 3.75–120 μg mL−1 for dihydroartemisinic acid and artemisinic acid. All validation indices were satisfactory, showing the method to be robust, quick, sensitive and adequate for a range of applications including high throughput (HTP) analysis.
Item Type: | Journal Article | ||||
---|---|---|---|---|---|
Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry Faculty of Science, Engineering and Medicine > Engineering > Engineering Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) |
||||
Journal or Publication Title: | Journal of Pharmaceutical and Biomedical Analysis | ||||
Publisher: | Elsevier BV | ||||
ISSN: | 0731-7085 | ||||
Official Date: | October 2013 | ||||
Dates: |
|
||||
Volume: | Volume 84 | ||||
Page Range: | pp. 269-277 | ||||
DOI: | 10.1016/j.jpba.2013.06.025 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published |
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |