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A rapid method for the determination of artemisinin and its biosynthetic precursors in Artemisia annua L. crude extracts

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Suberu, John O., Song, Lijiang, Slade, Susan E., Sullivan, Neil F., Barker, Guy C. and Lapkin, Alexei (2013) A rapid method for the determination of artemisinin and its biosynthetic precursors in Artemisia annua L. crude extracts. Journal of Pharmaceutical and Biomedical Analysis, Volume 84 . pp. 269-277. doi:10.1016/j.jpba.2013.06.025

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Official URL: http://dx.doi.org/10.1016/j.jpba.2013.06.025

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Abstract

A rapid high-pressure liquid chromatography (HPLC) tandem mass spectrometry (TQD) method for the determination of artemisinin, 9-epi-artemisinin, artemisitene, dihydroartemisinic acid, artemisinic acid and arteannuin B in Artemisia annua extracts is described. Detection and quantification of 9-epi-artemisinin in crude extracts are reported for the first time. In this method all six metabolites are resolved and eluted within 6 min with minimal sample preparation. A recovery of between 96.25% and 103.59% was obtained for all metabolites analysed and the standard curves were linear (r2 > 0.99) over the concentration range of 0.15–10 μg mL−1 for artemisinin, 9-epi-artemisinin, artemisitene and arteannuin B, and the range of 3.75–120 μg mL−1 for dihydroartemisinic acid and artemisinic acid. All validation indices were satisfactory, showing the method to be robust, quick, sensitive and adequate for a range of applications including high throughput (HTP) analysis.

Item Type: Journal Article
Divisions: Faculty of Science > Chemistry
Faculty of Science > Engineering
Faculty of Science > Life Sciences (2010- )
Journal or Publication Title: Journal of Pharmaceutical and Biomedical Analysis
Publisher: Elsevier BV
ISSN: 0731-7085
Official Date: October 2013
Dates:
DateEvent
October 2013Published
Volume: Volume 84
Page Range: pp. 269-277
DOI: 10.1016/j.jpba.2013.06.025
Status: Peer Reviewed
Publication Status: Published

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