Suberu, John O., Song, Lijiang, Slade, Susan E., Sullivan, Neil F., Barker, Guy C. and Lapkin, Alexei (2013) A rapid method for the determination of artemisinin and its biosynthetic precursors in Artemisia annua L. crude extracts. Journal of Pharmaceutical and Biomedical Analysis, Volume 84 . pp. 269-277. doi:10.1016/j.jpba.2013.06.025

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Abstract

A rapid high-pressure liquid chromatography (HPLC) tandem mass spectrometry (TQD) method for the determination of artemisinin, 9-epi-artemisinin, artemisitene, dihydroartemisinic acid, artemisinic acid and arteannuin B in Artemisia annua extracts is described. Detection and quantification of 9-epi-artemisinin in crude extracts are reported for the first time. In this method all six metabolites are resolved and eluted within 6 min with minimal sample preparation. A recovery of between 96.25% and 103.59% was obtained for all metabolites analysed and the standard curves were linear (r2 > 0.99) over the concentration range of 0.15–10 μg mL−1 for artemisinin, 9-epi-artemisinin, artemisitene and arteannuin B, and the range of 3.75–120 μg mL−1 for dihydroartemisinic acid and artemisinic acid. All validation indices were satisfactory, showing the method to be robust, quick, sensitive and adequate for a range of applications including high throughput (HTP) analysis.

Item Type:	Journal Article
Divisions:	Faculty of Science > Chemistry Faculty of Science > Engineering Faculty of Science > Life Sciences (2010- )
Journal or Publication Title:	Journal of Pharmaceutical and Biomedical Analysis
Publisher:	Elsevier BV
ISSN:	0731-7085
Official Date:	October 2013
Dates:	Date Event October 2013 Published
Volume:	Volume 84
Page Range:	pp. 269-277
DOI:	10.1016/j.jpba.2013.06.025
Status:	Peer Reviewed
Publication Status:	Published

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