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Cloning and expression analysis of beta-isopropylmalate dehydrogenase from potato
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Jackson, Stephen D., Sonnewald, Uwe and Willmitzer, Lothar (1993) Cloning and expression analysis of beta-isopropylmalate dehydrogenase from potato. Molecular & General Genetics, Volume 236 (Number 2-3). pp. 309-314. doi:10.1007/BF00277127 ISSN 0026-8925.
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Official URL: http://dx.doi.org/10.1007/BF00277127
Abstract
A full-length cDNA clone for β-isopropylmalate dehydrogenase from potato has been isolated and sequenced. The open reading frame is 1071 by in length encoding a protein of 357 amino acids which includes a 29 amino acid, putative chloroplastic transit peptide. The amino acid sequence shows 33.3% and 28.6% identity to β-isopropylmalate dehydrogenases from rape and Bacillus subtilis, respectively. Southern analysis shows that the gene is present in low copy number in potato, and in single copy in tomato and Arabidopsis. The gene is expressed in all tissues of the potato plant and its expression is increased by leucine, and leucine plus threonine, in contrast to the situation in yeast and prokaryotes. The gene is also induced by sucrose in a manner similar to that seen with genes involved in carbohydrate metabolism, which indicates that there may be some interaction at the transcriptional level between genes involved in carbon and nitrogen metabolism.
Item Type: | Journal Article | ||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
Journal or Publication Title: | Molecular & General Genetics | ||||
Publisher: | Springer | ||||
ISSN: | 0026-8925 | ||||
Official Date: | January 1993 | ||||
Dates: |
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Volume: | Volume 236 | ||||
Number: | Number 2-3 | ||||
Page Range: | pp. 309-314 | ||||
DOI: | 10.1007/BF00277127 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access |
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