The Library
High mass accuracy analytical applications of Fourier transform ion cyclotron resonance mass spectrometry
Tools
Tools
Tools
Wills, Rebecca Helen (2014) High mass accuracy analytical applications of Fourier transform ion cyclotron resonance mass spectrometry. PhD thesis, University of Warwick.
|
PDF
WRAP_THESIS_Wills_2014.pdf - Submitted Version - Requires a PDF viewer. Download (20Mb) | Preview |
Official URL: http://webcat.warwick.ac.uk/record=b2716474~S1
Abstract
The performance capabilities of Fourier transform ion cyclotron resonance (FTICR)
mass spectrometry are higher than any other type of mass spectrometer, making this
technique suitable for a range of analytical applications. Here, FTICR mass
spectrometry has been used for the structural analysis of polyketides and nonribosomal
peptides, and in the identification of peptide binding sites of ruthenium(II)
arene anticancer complexes. In both these applications, methods have been
developed involving complementary tandem mass spectrometry techniques,
specifically collision activated dissociation (CAD), electron induced dissociation
(EID), and electron capture dissociation. In particular, CAD and EID have been
shown to be effective in the structural characterisation of polyketides, with a method
developed for distinguishing between two isomers of the polyketide lasalocid A.
This method has been optimised and extended for application to non-ribosomal
peptides enabling detailed structural information to be obtained with very high
accuracy. Using CAD and ECD has enabled the identification of amino acids
involved in binding ruthenium(II) complexes. Binding to phenylalanine and
glutamic acid was observed in this work for the first time; coordination by histidine
and methionine was also observed and is in agreement with previous work. Overall,
new methods for highly accurate structural characterisation and binding site
identification have been successfully designed and implemented.
| Item Type: | Thesis or Dissertation (PhD) | ||||
|---|---|---|---|---|---|
| Subjects: | Q Science > QD Chemistry | ||||
| Library of Congress Subject Headings (LCSH): | Ion cyclotron resonance spectrometry, Fourier transform spectroscopy | ||||
| Official Date: | January 2014 | ||||
| Dates: |
|
||||
| Institution: | University of Warwick | ||||
| Theses Department: | Department of Chemistry | ||||
| Thesis Type: | PhD | ||||
| Publication Status: | Unpublished | ||||
| Supervisor(s)/Advisor: | O'Connor, Peter | ||||
| Extent: | xxiii, 257 leaves : illustrations. | ||||
| Language: | eng |
Request changes or add full text files to a record
Repository staff actions (login required)
 |
View Item |
Downloads
Downloads per month over past year
