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TGF 1 and SGK1-sensitive store-operated Ca2+ entry and Orai1 expression in endometrial Ishikawa cells
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Schmidt, S., Schneider, S., Yang, W., Liu, G., Schmidt, E. - M., Schmid, E., Mia, S., Brucker, S., Stournaras, C., Wallwiener, D., Brosens, Jan J. and Lang, F. (2013) TGF 1 and SGK1-sensitive store-operated Ca2+ entry and Orai1 expression in endometrial Ishikawa cells. Molecular Human Reproduction, Volume 20 (Number 2). pp. 139-147. doi:10.1093/molehr/gat066 ISSN 1360-9947.
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Official URL: http://dx.doi.org/10.1093/molehr/gat066
Abstract
The serum-and-glucocorticoid-inducible-kinase-1 (SGK1) is ubiquitously expressed and under genomic control by cell stress, hormones and further mediators. A most powerful stimulator of SGK1 expression is transforming growth factor TGFβ1. SGK1 is activated by insulin and growth factors via phosphatidylinositol-3-kinase and the 3-phosphoinositide-dependent kinase PDK1. As shown recently, SGK1 increases the store-operated Ca2+ entry (SOCE), which is accomplished by the pore-forming ion channel unit Orai. Most recent observations further revealed that SGK1 plays a critical role in the regulation of fertility. SGK1 is up-regulated in the luminal epithelium of women with unexplained infertility but down-regulated in decidualizing stromal cells of patients with recurrent pregnancy loss. The present study explored whether Orai1 is expressed in endometrium and sensitive to regulation by SGK1 and/or TGFβ1. To this end, Orai1 protein abundance was determined by western blotting and SOCE by fura-2 fluorescence. As a result, Orai1 was expressed in human endometrium and in human endometrial Ishikawa cells. Orai1 expression and SOCE in Ishikawa cells were increased by transfection with constitutively active S422DSGK1 but not by transfection with inactive K127NSGK1. The difference of SOCE between S422DSGK1 and K127NSGK1-transfected cells was virtually abrogated in the presence of Orai1 inhibitor 2-aminoethoxydiphenyl borate (2-APB, 50 µM). Similar to S422DSGK1 transfection TGFβ1 treatment up-regulated both Orai1 protein abundance and SOCE. In conclusion, Orai1 is expressed in the human endometrium and is up-regulated by SGK1 and TGFβ1. The present observations thus uncover a novel element in SGK1-sensitive regulation of endometrial cells.
| Item Type: | Journal Article | ||||||||||
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| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Translational & Experimental Medicine > Reproductive Health ( - until July 2016) Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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| Journal or Publication Title: | Molecular Human Reproduction | ||||||||||
| Publisher: | Oxford University Press | ||||||||||
| ISSN: | 1360-9947 | ||||||||||
| Official Date: | 16 September 2013 | ||||||||||
| Dates: |
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| Volume: | Volume 20 | ||||||||||
| Number: | Number 2 | ||||||||||
| Page Range: | pp. 139-147 | ||||||||||
| DOI: | 10.1093/molehr/gat066 | ||||||||||
| Status: | Peer Reviewed | ||||||||||
| Publication Status: | Published | ||||||||||
| Access rights to Published version: | Restricted or Subscription Access |
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