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Utility of a fecal real-time PCR protocol for detection of Mycobacterium bovisInfection in African buffalo (syncerus caffer)
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Roug, Annette, Geoghegan, Claire, Wellington, E. M. H., Miller, Woutrina A., Travis, Emma Rachel, Porter, David, Cooper, David, Clifford, Deana L., Mazet, Jonna A. K. and Parsons, Sven (2014) Utility of a fecal real-time PCR protocol for detection of Mycobacterium bovisInfection in African buffalo (syncerus caffer). Journal of Wildlife Diseases , Volume 50 (Number 1). pp. 140-142. doi:10.7589/2013-07-159
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Official URL: http://dx.doi.org/10.7589/2013-07-159
Abstract
A real-time PCR protocol for detecting Mycobacterium bovis in feces was evaluated in bovine tuberculosis–infected African buffalo (Syncerus caffer). Fecal samples spiked with 1.42×103 cells of M. bovis culture/g and Bacille Calmette–Guérin standards with 1.58×101 genome copies/well were positive by real-time PCR but all field samples were negative.
| Item Type: | Journal Item | ||||
|---|---|---|---|---|---|
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
| Journal or Publication Title: | Journal of Wildlife Diseases | ||||
| Publisher: | Wildlife Disease Association, Inc. | ||||
| ISSN: | 0090-3558 | ||||
| Official Date: | January 2014 | ||||
| Dates: |
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| Volume: | Volume 50 | ||||
| Number: | Number 1 | ||||
| Page Range: | pp. 140-142 | ||||
| DOI: | 10.7589/2013-07-159 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access |
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