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Utility of a fecal real-time PCR protocol for detection of Mycobacterium bovisInfection in African buffalo (syncerus caffer)

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Roug, Annette, Geoghegan, Claire, Wellington, E. M. H., Miller, Woutrina A., Travis, Emma Rachel, Porter, David, Cooper, David, Clifford, Deana L., Mazet, Jonna A. K. and Parsons, Sven (2014) Utility of a fecal real-time PCR protocol for detection of Mycobacterium bovisInfection in African buffalo (syncerus caffer). Journal of Wildlife Diseases , Volume 50 (Number 1). pp. 140-142. doi:10.7589/2013-07-159

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Official URL: http://dx.doi.org/10.7589/2013-07-159

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Abstract

A real-time PCR protocol for detecting Mycobacterium bovis in feces was evaluated in bovine tuberculosis–infected African buffalo (Syncerus caffer). Fecal samples spiked with 1.42×103 cells of M. bovis culture/g and Bacille Calmette–Guérin standards with 1.58×101 genome copies/well were positive by real-time PCR but all field samples were negative.

Item Type: Journal Item
Divisions: Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- )
Journal or Publication Title: Journal of Wildlife Diseases
Publisher: Wildlife Disease Association, Inc.
ISSN: 0090-3558
Official Date: January 2014
Dates:
DateEvent
January 2014Published
Volume: Volume 50
Number: Number 1
Page Range: pp. 140-142
DOI: 10.7589/2013-07-159
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Restricted or Subscription Access

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