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Rapid generation of a well-matched vaccine seed from a modern influenza A virus primary isolate without recourse to eggs

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Hartgroves, L. C. S., Koudstaal, W., McLeod, C., Moncorge, O., Thompson, C. I., Ellis, J., Bull, C., Havenga, M. J. E., Goudsmit, J. and Barclay, W. S. (2010) Rapid generation of a well-matched vaccine seed from a modern influenza A virus primary isolate without recourse to eggs. Vaccine, Vol.28 (No.17). pp. 2973-2979. doi:10.1016/j.vaccine.2010.02.012 ISSN 0264-410X.

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Official URL: http://dx.doi.org/10.1016/j.vaccine.2010.02.012

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Abstract

Most influenza vaccines are produced in chicken eggs but recent human influenza strains often do not grow well in this substrate. The PER.C6 (R) cell line is an alternative platform for vaccine production. Here we demonstrate that PER.C6 cells faithfully propagate recent clinical isolates, without selecting for mutations in the HA gene. PER.C6 cells support the rescue of recombinant influenza viruses from cDNA. We used sequence data from a surveillance programme to generate a PR8-based seed virus with the HA and NA of a contemporary circulating H3N2 human strain, A/England/611/07 (E611) that did not itself grow in eggs. We engineered mutations that affected receptor-binding, G186V or L194P, into the E611 HA gene. Whilst the L194P mutation conferred efficient growth in eggs, G186V did not. The L194P mutation was also spontaneously selected during egg propagation of E611/PR8 7:1 recombinant virus. This suggests generation of a single recombinant vaccine seed might satisfy manufacturers that utilize either eggs or cells for vaccine production. (C) 2010 Elsevier Ltd. All rights reserved.

Item Type: Journal Article
Subjects: Q Science > QR Microbiology > QR180 Immunology
R Medicine
Divisions: Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) > Biological Sciences ( -2010)
Journal or Publication Title: Vaccine
Publisher: Elsevier Ltd.
ISSN: 0264-410X
Official Date: 9 April 2010
Dates:
DateEvent
9 April 2010Published
Volume: Vol.28
Number: No.17
Number of Pages: 7
Page Range: pp. 2973-2979
DOI: 10.1016/j.vaccine.2010.02.012
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Restricted or Subscription Access
Funder: Sanofi-Pasteur, Crucell Holland B.V

Data sourced from Thomson Reuters' Web of Knowledge

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