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Structural analysis of prion proteins by means of drift cell and traveling wave ion mobility mass spectrometry
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Hilton, Gillian R., Thalassinos, Konstantinos, Grabenauer, Megan, Sanghera, Narinder, Slade, Susan E., Wyttenbach, Thomas, Robinson, Philip J., Pinheiro, Teresa J. T., Bowers, Michael T. and Scrivens, James H. (2010) Structural analysis of prion proteins by means of drift cell and traveling wave ion mobility mass spectrometry. In: Asilomar Conference on Ion Spectroscopy, Pacific Grove, CA, October 16-20, 2009. Published in: Journal of The American Society for Mass Spectrometry, Vol.21 (No.5). pp. 845-854. doi:10.1016/j.jasms.2010.01.017 ISSN 1044-0305.
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Official URL: http://dx.doi.org/10.1016/j.jasms.2010.01.017
Abstract
The prion protein (PrP) is implicitly involved in the pathogenesis of transmissible spongiform encephalopathies (TSEs). The conversion of normal cellular PrP (PrPC), a protein that is predominantly alpha-helical, to a beta-sheet-rich isoform (PrPSc), which has a propensity to aggregate, is the key molecular event in prion diseases. During its short life span, PrP can experience two different: pH environments; a mildly acidic environment, whilst cycling within the cell, and a neutral pH when it is glycosyl phosphatidylinositol (GPI)-anchored to the cell membrane. Ion mobility (IM) combined with mass spectrometry has been employed to differentiate between two conformational isoforms of recombinant Syrian hamster prion protein (SHaPrP). The recombinant proteins studied were a-helical SHaPrP(90-231) and beta-sheet-rich SHaPrP(90-231) at pH 5.5 and pH 7.0. The recombinant proteins have the same nominal mass-to-charge ratio (m/z) but differ in their secondary and tertiary structures. A comparison of traveling-wave (T-Wave) ion mobility and drift cell ion mobility (DCIM) mass spectrometry estimated and absolute cross-sections showed an excellent agreement between the two techniques. The use of T-Wave ion mobility as a shape-selective separation technique enabled differentiation between the estimated cross-sections and arrival time distributions (ATDs) of alpha-helical SHaPrP(90-231) and beta-sheet-rich SHaPrP(90-231) at pH 5.5. No differences in cross-section or ATD profiles were observed between the protein isoforms at pH 7.0. The findings have potential implications for a new ante-mortem screening assay, in bodily fluids, for prion misfolding diseases such as TSEs. (J Am Soc Mass Spectrom 2010, 21, 845-854) (C) 2010 American Society for Mass Spectrometry
| Item Type: | Conference Item (Paper) | ||||
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| Subjects: | Q Science > QD Chemistry Q Science > QC Physics |
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
| Journal or Publication Title: | Journal of The American Society for Mass Spectrometry | ||||
| Publisher: | Springer New York LLC | ||||
| ISSN: | 1044-0305 | ||||
| Official Date: | May 2010 | ||||
| Dates: |
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| Volume: | Vol.21 | ||||
| Number: | No.5 | ||||
| Number of Pages: | 10 | ||||
| Page Range: | pp. 845-854 | ||||
| DOI: | 10.1016/j.jasms.2010.01.017 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Conference Paper Type: | Paper | ||||
| Title of Event: | Asilomar Conference on Ion Spectroscopy | ||||
| Type of Event: | Conference | ||||
| Location of Event: | Pacific Grove, CA | ||||
| Date(s) of Event: | October 16-20, 2009 |
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