Tibbles, Katherine L. (2013) Regulation of Clb1 during meiosis in saccharomyces cerevisiae. PhD thesis, University of Warwick.

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Abstract

Meiosis is a specialised form of cell division in which diploid cells divide to form four non-identical spores containing half the genetic complement of the parent. During this cell division program, much of the usual machinery regulating cell division is put to alternate use to allow the cells to undergo an extra round of division without an intervening phase of DNA synthesis. In particular, the end of the first division, meiosis I, must be regulated differently than the end of the mitotic division. We used the model organism Saccharomyces cerevisiae to determine some of these differences in regulation.
The cell division program is driven by the sequential association of cyclins with the CDK (cyclin dependent kinase), leading to waves of kinase activity. Exit from mitosis requires the downregulation of CDK activity, and is coordinated by two signalling networks, the FEAR (Cdc14 Early Anaphase Release) network and the MEN (Mitotic Exit Network). Both networks initiate the release of the phosphatase Cdc14 from its inhibitor, Net1, to counter CDK activity. Exit from meiosis I similarly relies on Cdc14 activity, but is driven only by the FEAR network.
Experimental results showed that the phosphorylation state and subcellular localisation of the meiotic cyclin, C1b1, are altered in meiosis I. We investigated this relationship and aimed to determine the kinase responsible. We used modelling techniques to explore several rationales for the specific regulation of C1b1. We examined the functional significance of C1b1 localisation, using localisation mutants, and made an investigation into Cdc14 release in meiosis I.

Item Type:	Thesis (PhD)
Subjects:	Q Science > QH Natural history > QH301 Biology
Library of Congress Subject Headings (LCSH):	Meiosis, Saccharomyces cerevisiae, Cyclin-dependent kinases
Official Date:	August 2013
Dates:	Date Event August 2013 Submitted
Institution:	University of Warwick
Theses Department:	Systems Biology Doctoral Training Centre
Thesis Type:	PhD
Publication Status:	Unpublished
Supervisor(s)/Advisor:	Arumugam, Prakash; Novak, Bela; Krishnanunni, V. P.
Sponsors:	Engineering and Physical Sciences Research Council (EPSRC); Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC)
Extent:	xv, 233 leaves : illustrations, charts.
Language:	eng

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