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Charge remote fragmentation in electron capture and electron transfer dissociation
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Li, Xiaojuan, Lin, Cheng, Han, L. (Liang), Costello, Catherine E. and O'Connor, Peter B. (2010) Charge remote fragmentation in electron capture and electron transfer dissociation. Journal of The American Society for Mass Spectrometry, Vol.21 (No.4). pp. 646-656. doi:10.1016/j.jasms.2010.01.001
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Official URL: http://dx.doi.org/10.1016/j.jasms.2010.01.001
Abstract
Secondary fragmentations of three synthetic peptides (human alpha A crystallin peptide 1-11, the deamidated form of human beta B2 crystallin peptide 4-14, and amyloid beta peptide 25-35) were studied in both electron capture dissociation (ECD) and electron-transfer dissociation (ETD) mode. In ECD, in addition to c and z. ion formations, charge remote fragmentations (CRF) of z. ions were abundant, resulting in internal fragment formation or partial/entire side-chain losses from amino acids, sometimes several residues away from the backbone cleavage site, and to some extent multiple side-chain losses. The internal fragments were observed in peptides with basic residues located in the middle of the sequences, which was different from most tryptic peptides with basic residues located at the C-terminus. These secondary cleavages were initiated by hydrogen abstraction at the alpha-, beta-, or gamma-position of the amino acid side chain. In comparison, ETD generates fewer CRF fragments than ECD. This secondary cleavage study will facilitate ECD/ETD spectra interpretation, and help de novo sequencing and database searching. (J Am Soc Mass Spectrom 2010, 21, 646-656) (C) 2010 Published by Elsevier Inc. on behalf of American Society for Mass Spectrometry.
| Item Type: | Journal Article | ||||
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| Subjects: | Q Science > QD Chemistry Q Science > QC Physics |
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| Divisions: | Faculty of Science > Chemistry | ||||
| Library of Congress Subject Headings (LCSH): | Dissociation, Tandem mass spectrometry, Amino acids, Charge transfer, Fragmentation reactions, Peptides -- Synthesis | ||||
| Journal or Publication Title: | Journal of The American Society for Mass Spectrometry | ||||
| Publisher: | Springer New York LLC | ||||
| ISSN: | 1044-0305 | ||||
| Official Date: | April 2010 | ||||
| Dates: |
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| Volume: | Vol.21 | ||||
| Number: | No.4 | ||||
| Number of Pages: | 11 | ||||
| Page Range: | pp. 646-656 | ||||
| DOI: | 10.1016/j.jasms.2010.01.001 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access | ||||
| Funder: | National Institutes of Health (U.S.) (NIH), National Center for Research Resources (U.S.) (NCRR), National Heart, Lung, and Blood Institute (NHLBI), National Institute of General Medical Sciences (U.S.) (NIGMS), ACS Petroleum, Bruker Daltonics, MDS Sciex | ||||
| Grant number: | P41RR10888 (NIH/NCRR), N01HV28178 (NIH/NHLBI), R01GM078293 (NIH/NIGMS) |
Data sourced from Thomson Reuters' Web of Knowledge
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