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The use of fluorescence-activated cell sorting in studying plant development and environmental responses
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Carter, Anthony D., Bonyadi, Roxanna and Gifford, Miriam L. (2013) The use of fluorescence-activated cell sorting in studying plant development and environmental responses. The International Journal of Developmental Biology, Volume 57 . pp. 545-552. doi:10.1387/ijdb.130195mg
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Official URL: http://dx.doi.org/10.1387/ijdb.130195mg
Abstract
Fluorescence-Activated Cell Sorting (FACS) is a powerful tool that enables plant growth and development to be studied at the cellular level. Flow cytometry is used to isolate subpopulations of cells, such as those of specific cell types, or cells at particular developmental stages that have been marked with fluorescent proteins. Transgenic technology has given us the ability to generate plants that express fluorescent proteins, not just constitutively in particular cell types, but also dynamically in response to endogenous or external factors. By processing such transgenic lines with FACS, it is possible to isolate distinct populations of cells in a wide range of likely response states for further analysis. This is particularly useful for investigating biological mechanisms in plants because the control of growth and development is manifest at the cell type level. Furthermore, the specificity of the resulting data enables fine modelling of the transcriptional networks that exert systems-level control of the transcriptome; hence key regulators of responses and processes in the plant can be identified. In this review, the current state of the art for FACS methods in plants is explored by means of case studies of research in which cell sorting allowed us to make significant new discoveries.
| Item Type: | Journal Article | ||||
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| Subjects: | Q Science > QH Natural history > QH301 Biology Q Science > QH Natural history > QH426 Genetics Q Science > QK Botany S Agriculture > SB Plant culture |
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| Divisions: | Faculty of Science > Life Sciences (2010- ) | ||||
| Library of Congress Subject Headings (LCSH): | Roots (Botany), Arabidopsis, Cell separation, Flow cytometry, Plant genetic engineering | ||||
| Journal or Publication Title: | The International Journal of Developmental Biology | ||||
| Publisher: | Universidad del Pais Vasco * Servicio Editorial | ||||
| ISSN: | 0214-6282 | ||||
| Official Date: | 6 September 2013 | ||||
| Dates: |
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| Volume: | Volume 57 | ||||
| Page Range: | pp. 545-552 | ||||
| DOI: | 10.1387/ijdb.130195mg | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Open Access | ||||
| Funder: | Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Engineering and Physical Sciences Research Council (EPSRC), University of Warwick Postgraduate Research Scholarship, Stapley Educational Trust grant | ||||
| Grant number: | BB/H109502/1 (BBSRC) |
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