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A physiologically required G protein-coupled receptor (GPCR)-regulator of G protein signaling (RGS) interaction that compartmentalizes RGS activity

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Croft, Wayne D., Hills, Claire E., McCann, Eilish Clare, Bond, Michael, Esparza Franco, Manuel A., Bennett, Jeanette, Rand, D. A. (David A.), Davey, John and Ladds, Graham (2013) A physiologically required G protein-coupled receptor (GPCR)-regulator of G protein signaling (RGS) interaction that compartmentalizes RGS activity. Journal of Biological Chemistry, Volume 288 (Number 38). pp. 27327-27342. doi:10.1074/jbc.M113.497826

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Official URL: http://dx.doi.org/10.1074/jbc.M113.497826

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Abstract

G protein-coupled receptors (GPCRs) can interact with regulator of G protein signaling (RGS) proteins. However, the effects of such interactions on signal transduction and their physiological relevance have been largely undetermined. Ligand-bound GPCRs initiate by promoting exchange of GDP for GTP on the Gα subunit of heterotrimeric G proteins. Signaling is terminated by hydrolysis of GTP to GDP through intrinsic GTPase activity of the Gα subunit, a reaction catalyzed by RGS proteins. Using yeast as a tool to study GPCR signaling in isolation, we define an interaction between the cognate GPCR (Mam2) and RGS (Rgs1), mapping the interaction domains. This reaction tethers Rgs1 at the plasma membrane and is essential for physiological signaling response. In vivo quantitative data inform the development of a kinetic model of the GTPase cycle, which extends previous attempts by including GPCR-RGS interactions. In vivo and in silico data confirm that GPCR-RGS interactions can impose an additional layer of regulation through mediating RGS subcellular localization to compartmentalize RGS activity within a cell, thus highlighting their importance as potential targets to modulate GPCR signaling pathways.

Item Type: Journal Article
Divisions: Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- )
Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Microbiology & Infection
Faculty of Science, Engineering and Medicine > Research Centres > Warwick Systems Biology Centre
Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School
Journal or Publication Title: Journal of Biological Chemistry
Publisher: American Society for Biochemistry and Molecular Biology
ISSN: 0021-9258
Official Date: 30 July 2013
Dates:
DateEvent
30 July 2013Published
11 July 2013Submitted
Volume: Volume 288
Number: Number 38
Page Range: pp. 27327-27342
DOI: 10.1074/jbc.M113.497826
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Restricted or Subscription Access

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