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Recovery of a medieval Brucella melitensis genome using shotgun metagenomics
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Kay, Gemma L., Sergeant, Martin J., Giuffra, Valentina, Bandiera, Pasquale, Milanese, Marco, Bramanti, Barbara, Bianucci, Raffaella and Pallen, Mark J. (2014) Recovery of a medieval Brucella melitensis genome using shotgun metagenomics. mBio, Volume 5 (Number 4). Article number e01337-14. doi:10.1128/mBio.01337-14 ISSN 2150-7511.
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Official URL: http://dx.doi.org/10.1128/mBio.01337-14
Abstract
Shotgun metagenomics provides a powerful assumption-free approach to the recovery of pathogen genomes from contemporary and historical material. We sequenced the metagenome of a calcified nodule from the skeleton of a 14th-century middle-aged male excavated from the medieval Sardinian settlement of Geridu. We obtained 6.5-fold coverage of a Brucella melitensis genome. Sequence reads from this genome showed signatures typical of ancient or aged DNA. Despite the relatively low coverage, we were able to use information from single-nucleotide polymorphisms to place the medieval pathogen genome within a clade of B. melitensis strains that included the well-studied Ether strain and two other recent Italian isolates. We confirmed this placement using information from deletions and IS711 insertions. We conclude that metagenomics stands ready to document past and present infections, shedding light on the emergence, evolution, and spread of microbial pathogens.
Item Type: | Journal Article | ||||||||
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Subjects: | Q Science > QR Microbiology | ||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Microbiology & Infection Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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Library of Congress Subject Headings (LCSH): | Brucella melitensis -- Genetics, Brucella melitensis -- History, Metagenomics, Infection -- History | ||||||||
Journal or Publication Title: | mBio | ||||||||
Publisher: | American Society for Microbiology | ||||||||
ISSN: | 2150-7511 | ||||||||
Official Date: | 15 July 2014 | ||||||||
Dates: |
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Volume: | Volume 5 | ||||||||
Number: | Number 4 | ||||||||
Article Number: | Article number e01337-14 | ||||||||
DOI: | 10.1128/mBio.01337-14 | ||||||||
Status: | Peer Reviewed | ||||||||
Publication Status: | Published | ||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||
Date of first compliant deposit: | 27 December 2015 | ||||||||
Date of first compliant Open Access: | 27 December 2015 | ||||||||
Funder: | Sardinia (Italy), Warwick Medical School |
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