Skip to content Skip to navigation
University of Warwick
  • Study
  • |
  • Research
  • |
  • Business
  • |
  • Alumni
  • |
  • News
  • |
  • About

University of Warwick
Publications service & WRAP

Highlight your research

  • WRAP
    • Home
    • Search WRAP
    • Browse by Warwick Author
    • Browse WRAP by Year
    • Browse WRAP by Subject
    • Browse WRAP by Department
    • Browse WRAP by Funder
    • Browse Theses by Department
  • Publications Service
    • Home
    • Search Publications Service
    • Browse by Warwick Author
    • Browse Publications service by Year
    • Browse Publications service by Subject
    • Browse Publications service by Department
    • Browse Publications service by Funder
  • Help & Advice
University of Warwick

The Library

  • Login
  • Admin

Chromosome congression by CENP-E and CENP-Q dependent pathways

Tools
- Tools
+ Tools

Bancroft, James M. (2013) Chromosome congression by CENP-E and CENP-Q dependent pathways. PhD thesis, University of Warwick.

[img]
Preview
PDF
WRAP_THESIS_Bancroft_2013.pdf - Submitted Version - Requires a PDF viewer.

Download (75Mb) | Preview
Official URL: http://webcat.warwick.ac.uk/record=b2730711~S1

Request Changes to record.

Abstract

The timely and efficient movement of chromosomes to the spindle equator during mitosis is a prerequisite for accurate chromosome segregation. Recent work has shown that the majority of chromosomes are able to congress and biorientate almost instantaneously after nuclear envelope breakdown due to their position relative to the forming spindle. However, other mechanisms are required to facilitate the congression of chromosomes, which do not congress in this initial wave. Congression of these remaining chromosomes is mediated by multiple mechanisms including: (1) Kinetochore sliding along the microtubule lattice using the Kinesin-7 CENP-E, and (2) kinetochores biorientating near the pole and congressing through microtubule depolymerisation-coupled movement. Here, we show that the constitutive centromere associated network (CCAN) subunit CENP-Q is required for both mechanisms. CENP-Q is required to recruit CENP-E to kinetochores thus explaining the absence of lateral sliding in CENP-Q depleted cells. Because depletion or inhibition of the CENP-E motor does not affect depolymerisation-coupled pulling, we identify a CENP-E recruitment-independent role for CENP-Q in chromosome congression. Following congression we find that biorientated kinetochore movements require both CENP-Q and CENP-E dependent mechanisms. This suggests that as biorientated kinetochores congress they switch into a mode that requires CENP-E motor activity.

Item Type: Thesis or Dissertation (PhD)
Subjects: Q Science > QH Natural history
Library of Congress Subject Headings (LCSH): Mitosis
Official Date: December 2013
Dates:
DateEvent
December 2013Submitted
Institution: University of Warwick
Theses Department: Warwick Medical School
Thesis Type: PhD
Publication Status: Unpublished
Supervisor(s)/Advisor: McAinsh, Andrew D.
Extent: xv, 179, 15 leaves : illustrations
Language: eng

Request changes or add full text files to a record

Repository staff actions (login required)

View Item View Item

Downloads

Downloads per month over past year

View more statistics

twitter

Email us: wrap@warwick.ac.uk
Contact Details
About Us