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Differential expression of Toll-like receptors and inflammatory cytokines in ovine interdigital dermatitis and footrot
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Davenport, Rebecca, Heawood, Christopher, Sessford, Kate, Baker, Melissa, Baiker, Kerstin, Blacklaws, Barbara, Kaler, Jasmeet, Green, Laura E. and Tötemeyer, Sabine (2014) Differential expression of Toll-like receptors and inflammatory cytokines in ovine interdigital dermatitis and footrot. Veterinary Immunology and Immunopathology, Volume 161 (Number 1–2). pp. 90-98. doi:10.1016/j.vetimm.2014.07.007
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Official URL: http://dx.doi.org/10.1016/j.vetimm.2014.07.007
Abstract
Footrot is a common inflammatory bacterial disease affecting the health and welfare of sheep worldwide. The pathogenesis of footrot is complex and multifactorial. The primary causal pathogen is the anaerobic bacterium Dichelobacter nodosus, with Fusobacterium necrophorum also shown to play a key role in disease. Since immune-mediated pathology is implicated, the aim of this research was to investigate the role of the host response in interdigital dermatitis (ID) and footrot. We compared the expression of Toll-like receptors (TLR) and pro-inflammatory cytokines and the histological appearance of clinically normal in comparison to ID and footrot affected tissues. Severe ID and footrot were characterized by significantly increased transcript levels of pro-inflammatory cytokines TNFα and IL1β and the pattern recognition receptors TLR2 and TLR4 in the interdigital skin. This was reflected in the histopathological appearance, with ID and footrot presenting progressive chronic-active pododermatitis with a mixed lymphocytic and neutrophilic infiltration, gradually increasing from a mild form in clinically normal feet, to moderate in ID and to a focally severe form with frequent areas of purulence in footrot. Stimulation with F. necrophorum and/or D. nodosus extracts demonstrated that dermal fibroblasts, the resident cell type of the dermis, also contribute to the inflammatory response to footrot bacteria by increased expression of TNFα, IL1β and TLR2. Overall, ID and footrot lead to a local inflammatory response given that expression levels of TLRs and IL1β were dependent on the disease state of the foot not the animal.
| Item Type: | Journal Article | ||||||||||
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| Subjects: | Q Science > QR Microbiology > QR180 Immunology S Agriculture > SF Animal culture |
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| Divisions: | Faculty of Science > Life Sciences (2010- ) | ||||||||||
| Library of Congress Subject Headings (LCSH): | Veterinary Medicine, Footrot in sheep | ||||||||||
| Journal or Publication Title: | Veterinary Immunology and Immunopathology | ||||||||||
| Publisher: | Elsevier Science BV | ||||||||||
| ISSN: | 0165-2427 | ||||||||||
| Official Date: | 15 September 2014 | ||||||||||
| Dates: |
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| Date of first compliant deposit: | 27 December 2015 | ||||||||||
| Volume: | Volume 161 | ||||||||||
| Number: | Number 1–2 | ||||||||||
| Page Range: | pp. 90-98 | ||||||||||
| DOI: | 10.1016/j.vetimm.2014.07.007 | ||||||||||
| Status: | Peer Reviewed | ||||||||||
| Publication Status: | Published | ||||||||||
| Access rights to Published version: | Open Access | ||||||||||
| Funder: | University of Nottingham, Wellcome Trust (London, England), MSD Animal Health |
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