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Characterising the folding core of the cyclophilin A — cyclosporin A complex I : hydrogen exchange data and rigidity analysis
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Heal, Jack W., Römer, Rudolf A., Blindauer, Claudia A. and Freedman, R. B. (2014) Characterising the folding core of the cyclophilin A — cyclosporin A complex I : hydrogen exchange data and rigidity analysis. Biophysical Journal . (Submitted)
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Official URL: http://www.sciencedirect.com/science/journal/00063...
Abstract
The determination of a 'folding core' can help to provide insight into the structure, flexibility, mobility and dynamics, and hence, ultimately, function of a protein - a central concern of structural biology. Changes in the folding core upon ligand binding are of particular interest because they may be relevant to drug-induced functional changes. Cyclophilin A is a multi-functional ligand-binding protein and a significant drug target. It acts principally as an enzyme during protein folding, but also as the primary binding partner for the immunosuppressant drug cyclosporin A (CsA). Here, we have used hydrogen-deuterium exchange (HDX) NMR spectroscopy to determine the folding core of the CypA-CsA complex. We also use the rapid computational tool of rigidity analysis, implemented in FIRST, to determine a theoretical folding core of the complex. In addition we generate a theoretical folding core for the unbound protein and compare this with previously published HDX data. The FIRST method gives a good prediction of the HDX folding core, but we find that it is not yet sufficiently sensitive to predict the effects of ligand binding on CypA.
| Item Type: | Submitted Journal Article | ||||
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| Subjects: | Q Science > QC Physics Q Science > QH Natural history > QH301 Biology Q Science > QR Microbiology |
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| Divisions: | Faculty of Science > Chemistry Faculty of Science > Life Sciences (2010- ) Faculty of Science > Physics Faculty of Science > Centre for Scientific Computing |
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| Journal or Publication Title: | Biophysical Journal | ||||
| Publisher: | Biophysical Society | ||||
| ISSN: | 0006-3495 | ||||
| Official Date: | June 2014 | ||||
| Dates: |
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| Status: | Not Peer Reviewed | ||||
| Publication Status: | Submitted | ||||
| Publisher Statement: | submitted | ||||
| Description: | Combined with Characterising the folding core of the cyclophilin A — cyclosporin A complex II : improving folding core predictions by including mobility and later published as Heal, Jack W., Wells, Stephen A., Blindauer, Claudia A., Freedman, R. B. and Roemer, Rudolf A.. (2015) Characterization of folding cores in the cyclophilin A-cyclosporin A complex. Biophysical Journal, Volume 108 (Number 7). pp. 1739-1746. |
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| Funder: | Engineering and Physical Sciences Research Council (EPSRC) | ||||
| Grant number: | EP/F500378/1 | ||||
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