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Selective detection of epimeric pentose saccharides at physiological pH using a fluorescent receptor

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Gibson, Matthew I., Neres, João and Fullam, Elizabeth (2014) Selective detection of epimeric pentose saccharides at physiological pH using a fluorescent receptor. Carbohydrate research, Volume 391 . pp. 61-65. doi:10.1016/j.carres.2014.02.007 ISSN 1873-426X.

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Official URL: http://dx.doi.org/10.1016/j.carres.2014.02.007

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Abstract

Epimerisation between ribofuranose and arabinofuranose sugars is crucial in several biosynthetic pathways, but is typically challenging to monitor. Here, we have screened for fluorescent boronic acids that can be used as molecular probes for the specific detection of ribofuranose over arabinofuranose sugars in solution. We show excellent specificity of the fluorescent response of 3-biphenylboronic acid to ribofuranose at physiological pH. This provides a tool for in situ monitoring of carbohydrate modifying enzymes and provides a viable alternative to traditional radiolabelled assays.

Item Type: Journal Article
Divisions: Faculty of Science, Engineering and Medicine > Science > Chemistry
Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- )
Journal or Publication Title: Carbohydrate research
Publisher: Pergamon-Elsevier Science Ltd.
ISSN: 1873-426X
Official Date: 4 June 2014
Dates:
DateEvent
4 June 2014Published
15 February 2014Available
6 February 2014Accepted
22 July 2013Submitted
Volume: Volume 391
Page Range: pp. 61-65
DOI: 10.1016/j.carres.2014.02.007
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Restricted or Subscription Access

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