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Selective detection of epimeric pentose saccharides at physiological pH using a fluorescent receptor
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Gibson, Matthew I., Neres, João and Fullam, Elizabeth (2014) Selective detection of epimeric pentose saccharides at physiological pH using a fluorescent receptor. Carbohydrate research, Volume 391 . pp. 61-65. doi:10.1016/j.carres.2014.02.007
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Official URL: http://dx.doi.org/10.1016/j.carres.2014.02.007
Abstract
Epimerisation between ribofuranose and arabinofuranose sugars is crucial in several biosynthetic pathways, but is typically challenging to monitor. Here, we have screened for fluorescent boronic acids that can be used as molecular probes for the specific detection of ribofuranose over arabinofuranose sugars in solution. We show excellent specificity of the fluorescent response of 3-biphenylboronic acid to ribofuranose at physiological pH. This provides a tool for in situ monitoring of carbohydrate modifying enzymes and provides a viable alternative to traditional radiolabelled assays.
| Item Type: | Journal Article | ||||||||||
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) |
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| Journal or Publication Title: | Carbohydrate research | ||||||||||
| Publisher: | Pergamon-Elsevier Science Ltd. | ||||||||||
| ISSN: | 1873-426X | ||||||||||
| Official Date: | 4 June 2014 | ||||||||||
| Dates: |
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| Volume: | Volume 391 | ||||||||||
| Page Range: | pp. 61-65 | ||||||||||
| DOI: | 10.1016/j.carres.2014.02.007 | ||||||||||
| Status: | Peer Reviewed | ||||||||||
| Publication Status: | Published | ||||||||||
| Access rights to Published version: | Restricted or Subscription Access |
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