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Discrimination between bacterial phenotypes using glyco-nanoparticles and the impact of polymer coating on detection readouts
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Richards, Sarah-Jane, Fullam, Elizabeth, Besra, Gurdyal S. and Gibson, Matthew I. (2014) Discrimination between bacterial phenotypes using glyco-nanoparticles and the impact of polymer coating on detection readouts. Journal of Materials Chemistry B, Volume 2 (Number 11). pp. 1490-1498. doi:10.1039/c3tb21821j ISSN 2050-750X.
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Official URL: http://dx.doi.org/10.1039/C3TB21821J
Abstract
The identification and treatment of bacterial infections remains a major healthcare challenge, especially to ensure appropriate application of a limited spectrum of antibiotics. Here we describe a system capable of discriminating between different strains of Escherichia coli using multivalent, carbohydrate-functionalised, gold nanoparticles based on their different expression levels of the FimH adhesin. Upon binding of the glycosylated nanoparticles to FimH positive bacteria, the nanoparticles' optical properties change enabling the identification of bacteria strain. Comparison between direct conjugation, or via a linker, of the carbohydrate to the nanoparticle revealed significant effects on the performance of the detection system. Using a poly(ethylene glycol) spacer increased the stability, and specificity, of the glycosylated nanoparticles but also reduced aggregation upon bacterial binding. This prevented the well-known red-blue gold colour change, meaning spectrophometric, rather than optical, assessment methods were required. Using this method, FimH positive bacteria could be detected at approximately 1.5 × 107 colony forming units per mL.
Item Type: | Journal Article | ||||||||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) |
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Journal or Publication Title: | Journal of Materials Chemistry B | ||||||||||
Publisher: | R S C Publications | ||||||||||
ISSN: | 2050-750X | ||||||||||
Official Date: | 21 March 2014 | ||||||||||
Dates: |
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Volume: | Volume 2 | ||||||||||
Number: | Number 11 | ||||||||||
Page Range: | pp. 1490-1498 | ||||||||||
DOI: | 10.1039/c3tb21821j | ||||||||||
Status: | Peer Reviewed | ||||||||||
Publication Status: | Published | ||||||||||
Access rights to Published version: | Restricted or Subscription Access |
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