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Molecular and immunological characterization of profilin from mugwort pollen
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Wopfner, N., Willeroider, M., Hebenstreit, Daniel, Ree, R. van, Aalbers, M., Briza, P., Thalhamer, J., Ebner, C., Richter, K. and Ferreira, F. (2002) Molecular and immunological characterization of profilin from mugwort pollen. Biological Chemistry, Volume 383 (Number 11). pp. 1779-1789. doi:10.1515/BC.2002.199
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Official URL: http://dx.doi.org/10.1515/BC.2002.199
Abstract
In late summer in Europe, pollen of mugwort is one of the major sources of atopic allergens. No information about the complete molecular structure of any mugwort allergen has been published so far. Here we report the isolation and characterization of mugwort pollen cDNA clones coding for two isoforms of the panallergen profilin. Thirtysix percent of the mugwort allergic patients tested displayed IgE antibodies against natural and recombinant profilin, and no significant differences were observed in the IgEbinding properties of the isoforms. One profilin isoform was purified to homogeneity and detailed structural analysis indicated that the protein exists in solution as dimers and tetramers stabilized by sulfydryl and/or ionic interactions. Profilin monomers were detectable only after exposure of multimers to harsh denaturing conditions. Dimers and tetramers did not significantly differ in their ability to bind serum IgE from mugwort pollenallergic patients. However, oligomeric forms might have a higher allergenic potential than monomers because larger molecules would have additional epitopes for IgEmediated histamine release. Profilin isolated from mugwort pollen also formed multimers. Thus, oligomerization is not an artifact resulting from the recombinant production of the allergen. Inhibition experiments showed extensive IgE crossreactivity of recombinant mugwort profilin and profilin from various pollen and food extracts.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Subjects: | Q Science > QR Microbiology | ||||
| Divisions: | Faculty of Science > Life Sciences (2010- ) | ||||
| Library of Congress Subject Headings (LCSH): | Allergens, Gene mapping, Molecular cloning, Oligomerization | ||||
| Journal or Publication Title: | Biological Chemistry | ||||
| Publisher: | Walter de Gruyter & Co | ||||
| ISSN: | 1431-6730 | ||||
| Official Date: | June 2002 | ||||
| Dates: |
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| Volume: | Volume 383 | ||||
| Number: | Number 11 | ||||
| Page Range: | pp. 1779-1789 | ||||
| DOI: | 10.1515/BC.2002.199 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access | ||||
| Funder: | Austria. Bundesministerium für Wissenschaft und Forschung [Federal Ministry of Education, Science and Culture], Österreichische Nationalbank | ||||
| Grant number: | S8802-MED (BWF), S8808-MED (BWF), S8811-MED (BWF), 6927 (ON) |
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