The Library
TnpR encoded by an ISPpu12 isoform regulates transposition of two different ISL3-like insertion sequences in pseudomonas stutzeri after conjugative interaction
Tools
Christie-Oleza, Joseph Alexander, Nogales, B., Lalucat, J. and Bosch, R. (2010) TnpR encoded by an ISPpu12 isoform regulates transposition of two different ISL3-like insertion sequences in pseudomonas stutzeri after conjugative interaction. Journal of Bacteriology, Volume 192 (Number 5). pp. 1423-1432. doi:10.1128/JB.01336-09 ISSN 0021-9193.
Research output not available from this repository.
Request-a-Copy directly from author or use local Library Get it For Me service.
Official URL: http://dx.doi.org/10.1128/JB.01336-09
Abstract
Pseudomonas stutzeri AN10 has two ISL3-like insertion sequences (ISs). One of them has been recently described as ISPst9. In this study we show that the second IS, situated 4.5 kb upstream of ISPst9, is an isoform of ISPpu12 from Pseudomonas putida mt-2. Although both ISL3-like ISs are flanked by nearly identical (21/24 conserved residues) inverted repeats (IRs) and harbor similar transposases (93% amino acid identity), they differ in their accompanying genes. As described for ISPst9, the isoform of ISPpu12 also transposes by a conservative mechanism, forms circular double-stranded DNA (dsDNA) transposition intermediates, and is induced by interaction with the conjugative strain Escherichia coli S17-1λpir (conjugative interaction) but not with the nonconjugative E. coli DH5α. In fact, we demonstrate that ISPst9 transposition after conjugative interaction occurs only when ISPpu12 is present, indicating that ISPpu12 is upregulating transposition of both ISs under such conditions. We also demonstrate that this conjugative interaction-mediated induction of ISPpu12 is not exclusive to the P. stutzeri AN10 strain but is a more general phenomenon, at least in Pseudomonas. Mutation of TnpR, a MerR-like transcriptional regulator present in ISPpu12 but not in ISPst9, reduced the transcription of tnpA (ISPpu12 transposase-encoding gene) and decreased formation of circular dsDNA transposition intermediates after conjugative interaction. Complementation of the TnpR mutant restored the phenotype. In addition, the presence of TnpR in an ISPpu12-free genetic background did not induce ISPst9 after conjugative interaction. Thus, our results suggest that TnpR, after conjugative interaction, activates transcription of tnpA of ISPpu12. Then, TnpA of ISPpu12 would bind to IRs of both ISs, ISPpu12 and ISPst9, causing their transposition.
Item Type: | Journal Article | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||||
Journal or Publication Title: | Journal of Bacteriology | ||||||||||
Publisher: | American Society for Microbiology | ||||||||||
ISSN: | 0021-9193 | ||||||||||
Official Date: | March 2010 | ||||||||||
Dates: |
|
||||||||||
Volume: | Volume 192 | ||||||||||
Number: | Number 5 | ||||||||||
Page Range: | pp. 1423-1432 | ||||||||||
DOI: | 10.1128/JB.01336-09 | ||||||||||
Status: | Peer Reviewed | ||||||||||
Publication Status: | Published | ||||||||||
Access rights to Published version: | Restricted or Subscription Access |
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |