Skip to content Skip to navigation
University of Warwick
  • Study
  • |
  • Research
  • |
  • Business
  • |
  • Alumni
  • |
  • News
  • |
  • About

University of Warwick
Publications service & WRAP

Highlight your research

  • WRAP
    • Home
    • Search WRAP
    • Browse by Warwick Author
    • Browse WRAP by Year
    • Browse WRAP by Subject
    • Browse WRAP by Department
    • Browse WRAP by Funder
    • Browse Theses by Department
  • Publications Service
    • Home
    • Search Publications Service
    • Browse by Warwick Author
    • Browse Publications service by Year
    • Browse Publications service by Subject
    • Browse Publications service by Department
    • Browse Publications service by Funder
  • Help & Advice
University of Warwick

The Library

  • Login
  • Admin

In-depth analysis of exoproteomes from marine bacteria by shotgun liquid chromatography-tandem mass spectrometry : the Ruegeria pomeroyi DSS-3 case-study

Tools
- Tools
+ Tools

Christie-Oleza, Joseph Alexander and Armengaud, Jean (2010) In-depth analysis of exoproteomes from marine bacteria by shotgun liquid chromatography-tandem mass spectrometry : the Ruegeria pomeroyi DSS-3 case-study. Marine Drugs, Volume 8 (Number 8). pp. 2223-2239. doi:10.3390/md8082223

[img]
Preview
PDF
WRAP_1074326-lf-040814-christie-oleza_2010_marinedrugs.pdf - Published Version - Requires a PDF viewer.
Available under License Creative Commons Attribution.

Download (460Kb) | Preview
Official URL: http://dx.doi.org/10.3390/md8082223

Request Changes to record.

Abstract

Microorganisms secrete into their extracellular environment numerous compounds that are required for their survival. Many of these compounds could be of great interest for biotechnology applications and their genes used in synthetic biology design. The secreted proteins and the components of the translocation systems themselves can be scrutinized in-depth by the most recent proteomic tools. While the secretomes of pathogens are well-documented, those of non-pathogens remain largely to be established. Here, we present the analysis of the exoproteome from the marine bacterium Ruegeria pomeroyi DSS-3 grown in standard laboratory conditions. We used a shotgun approach consisting of trypsin digestion of the exoproteome, and identification of the resulting peptides by liquid chromatography coupled to tandem mass spectrometry. Three different proteins that have domains homologous to those observed in RTX toxins were uncovered and were semi-quantified as the most abundantly secreted proteins. One of these proteins clearly stands out from the catalogue, representing over half of the total exoproteome. We also listed many soluble proteins related to ABC and TRAP transporters implied in the uptake of nutrients. The Ruegeria pomeroyi DSS-3 case-study illustrates the power of the shotgun nano-LC-MS/MS strategy to decipher the exoproteome from marine bacteria and to contribute to environmental proteomics.

Item Type: Journal Article
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QR Microbiology
Divisions: Faculty of Science > Life Sciences (2010- )
Library of Congress Subject Headings (LCSH): Marine bacteria, Proteome -- genetics, Environmental proteomics
Journal or Publication Title: Marine Drugs
Publisher: M D P I
ISSN: 1660-3397
Official Date: 29 July 2010
Dates:
DateEvent
29 July 2010Published
28 July 2010Accepted
7 June 2010Submitted
Volume: Volume 8
Number: Number 8
Page Range: pp. 2223-2239
DOI: 10.3390/md8082223
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Open Access
Funder: Fundación Ramón Areces, France. Commissariat à l'énergie atomique (CEA), France. Ministèré des affaires étrangères, France. République Française (Partenariat Hubert Curien)

Request changes or add full text files to a record

Repository staff actions (login required)

View Item View Item

Downloads

Downloads per month over past year

View more statistics

twitter

Email us: wrap@warwick.ac.uk
Contact Details
About Us