The Library
Comparative proteogenomics of twelve roseobacter exoproteomes reveals different adaptive strategies among these marine bacteria
Tools
Christie-Oleza, Joseph Alexander, Pina-Villalonga, J. M., Bosch, R., Nogales, B. and Armengaud, J. (2012) Comparative proteogenomics of twelve roseobacter exoproteomes reveals different adaptive strategies among these marine bacteria. Molecular & Cellular Proteomics, Volume 11 (Number 2). Article number M111.013110. doi:10.1074/mcp.M111.013110 ISSN 1535-9476.
Research output not available from this repository.
Request-a-Copy directly from author or use local Library Get it For Me service.
Official URL: http://dx.doi.org/10.1074/mcp.M111.013110
Abstract
Roseobacters are generalist bacteria abundantly found in the oceans. Because little is known on how marine microorganisms interact in association or competition, we focused our attention on the microbial exoproteome, a key component in their interaction with extracellular milieu. Here we present a comparative analysis of the theoretically encoded exoproteome of twelve members of the Roseobacter group validated by extensive comparative proteogenomics. In silico analysis revealed that 30% of the encoded proteome of these microorganisms could be exported. The ratio of the different protein categories varied in accordance to the ecological distinctness of each strain, a trait reinforced by quantitative proteomics data. Despite the interspecies variations found, the most abundantly detected proteins by shotgun proteomics were from transporter, adhesion, motility, and toxin-like protein categories, defining four different plausible adaptive strategies within the Roseobacter group. In some strains the toxin-secretion strategy was over-represented with repeats-in-toxin-like proteins. Our results show that exoproteomes strongly depend on bacterial trophic strategy and can slightly change because of culture conditions. Simulated natural conditions and the effect of the indigenous microbial community on the exoproteome of Ruegeria pomeroyi DSS-3 were also assayed. Interestingly, we observed a significant depletion of the toxin-like proteins usually secreted by R. pomeroyi DSS-3 when grown in presence of a natural community sampled from a Mediterranean Sea port. The significance of this specific fraction of the exoproteome is discussed.
Item Type: | Journal Article | ||||||
---|---|---|---|---|---|---|---|
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||
Journal or Publication Title: | Molecular & Cellular Proteomics | ||||||
Publisher: | American Society for Biochemistry and Molecular Biology | ||||||
ISSN: | 1535-9476 | ||||||
Official Date: | 28 November 2012 | ||||||
Dates: |
|
||||||
Volume: | Volume 11 | ||||||
Number: | Number 2 | ||||||
Article Number: | Article number M111.013110 | ||||||
DOI: | 10.1074/mcp.M111.013110 | ||||||
Status: | Peer Reviewed | ||||||
Publication Status: | Published |
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |