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Direct regulation of the NADPH oxidase RBOHD by the PRR-associated kinase BIK1 during plant immunity
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Kadota, Yasuhiro, Sklenar, Jan, Derbyshire, Paul, Stransfeld, Lena, Asai, Shuta, Ntoukakis, Vardis, Jones, Jonathan D. G., Shirasu, Ken, Menke, Frank, Jones, Alexandra M. and Zipfel, Cyril (2014) Direct regulation of the NADPH oxidase RBOHD by the PRR-associated kinase BIK1 during plant immunity. Molecular Cell, Volume 54 (Number 1). pp. 43-55. doi:10.1016/j.molcel.2014.02.021 ISSN 1097-2765.
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Official URL: http://dx.doi.org/10.1016/j.molcel.2014.02.021
Abstract
The rapid production of reactive oxygen species (ROS) burst is a conserved signaling output in immunity across kingdoms. In plants, perception of pathogen-associated molecular patterns (PAMPs) by surface-localized pattern recognition receptors (PRRs) activates the NADPH oxidase RBOHD by hitherto unknown mechanisms. Here, we show that RBOHD exists in complex with the receptor kinases EFR and FLS2, which are the PRRs for bacterial EF-Tu and flagellin, respectively. The plasma-membrane-associated kinase BIK1, which is a direct substrate of the PRR complex, directly interacts with and phosphorylates RBOHD upon PAMP perception. BIK1 phosphorylates different residues than calcium-dependent protein kinases, and both PAMP-induced BIK1 activation and BIK1-mediated phosphorylation of RBOHD are calcium independent. Importantly, phosphorylation of these residues is critical for the PAMP-induced ROS burst and antibacterial immunity. Our study reveals a rapid regulatory mechanism of a plant RBOH, which occurs in parallel with and is essential for its paradigmatic calcium-based regulation.
Item Type: | Journal Article | ||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
Journal or Publication Title: | Molecular Cell | ||||
Publisher: | Cell Press | ||||
ISSN: | 1097-2765 | ||||
Official Date: | 10 April 2014 | ||||
Dates: |
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Volume: | Volume 54 | ||||
Number: | Number 1 | ||||
Page Range: | pp. 43-55 | ||||
DOI: | 10.1016/j.molcel.2014.02.021 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access |
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