Speulman, Elly, Bouchez, David, Holub, E. B. and Beynon, Jim (1998) Disease resistance gene homologs correlate with disease resistance loci of Arabidopsis thaliana. The Plant Journal, Volume 14 (Number 4). pp. 467-474. doi:10.1046/j.1365-313X.1998.00138.x

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Abstract

The disease resistance genes RPS2 of Arabidopsis and N of tobacco, among other recently cloned resistance genes, share several conserved sequences. Degenerate oligonucleotide primers, based on conserved sequences in the nucleotide binding site (NBS) and a weak hydrophobic domain of RPS2 and N, were used to amplify homologous sequences from Arabidopsis thaliana. Amplification products were obtained that were similar in sequence to the disease resistance genes RPS2, RPM1, N and L6. The Arabidopsis CIC-YAC library was used to identify the position of the disease resistance homologs on the Arabidopsis genome. Their map positions could be correlated with the disease resistance loci RPS5, RAC1, RPP9, CAR1, RPP7, RPW2, RPP1, RPP10, RPP14, RPP5, RPP4, RPS2, RPW6, HRT, RPS4, RPP8, RPP21, RPP22, RPP23, RPP24 and TTR1. This method was therefore not only successful in the identification of sequences located within gene clusters that are involved in disease resistance, but could also contribute to the cloning of disease resistance genes from Arabidopsis.

Item Type:	Journal Article
Divisions:	Faculty of Science > Life Sciences (2010- )
Journal or Publication Title:	The Plant Journal
Publisher:	Wiley-Blackwell Publishing Ltd.
ISSN:	0960-7412
Official Date:	1998
Dates:	Date Event 1998 UNSPECIFIED
Volume:	Volume 14
Number:	Number 4
Page Range:	pp. 467-474
DOI:	10.1046/j.1365-313X.1998.00138.x
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access

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