Aarts, Mark G. M., Lintel Hekkert, Bas te, Holub, E. B., Beynon, Jim, Stiekema, Willem J. and Pereira, Andy (1998) Identification of R-Gene homologous DNA fragments genetically linked to disease resistance loci in Arabidopsis thaliana. Molecular Plant-Microbe Interactions, Volume 11 (Number 4). pp. 251-258. doi:10.1094/MPMI.1998.11.4.251

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Abstract

Disease resistance in plants is a desirable economic trait. A number of disease resistance genes from various plant species have been cloned so far. The gene products of some of these can be distinguished by the presence of an N-terminal nucleotide binding site and a C-terminal stretch of leucine-rich repeats. Although these gene products are structurally related, the DNA sequences are poorly conserved. Only parts of the nucleotide binding site share enough DNA identity to design primers for polymerase chain reaction amplification of related DNA sequences. Such primers were used to amplify different resistance-gene-like (RGL) DNA fragments from Arabidopsis thaliana accessions Landsberg erecta and Columbia. Almost all cloned DNA fragments were genetically closely linked with known disease resistance loci. Most RGL fragments were found in a clustered or dispersed multi-copy sequence organization, supporting the supposed correlation of RGL sequences and disease resistance loci.

Item Type:	Journal Article
Divisions:	Faculty of Science > Life Sciences (2010- )
Journal or Publication Title:	Molecular Plant-Microbe Interactions
Publisher:	American Phytopathological Society
ISSN:	0894-0282
Official Date:	April 1998
Dates:	Date Event April 1998 Published
Volume:	Volume 11
Number:	Number 4
Page Range:	pp. 251-258
DOI:	10.1094/MPMI.1998.11.4.251
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access

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