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Conformational switching in PolyGln amyloid fibrils resulting from a single amino acid insertion
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Huang, Rick K., Baxa, Ulrich, Aldrian, Gudrun, Ahmed, Abdullah B., Wall, Joseph S., Mizuno, Naoko, Antzutkin, Oleg N., Steven, Alasdair C. and Kajava, Andrey V. (2014) Conformational switching in PolyGln amyloid fibrils resulting from a single amino acid insertion. Biophysical Journal, 106 (10). pp. 2134-2142. doi:10.1016/j.bpj.2014.03.047 ISSN 0006-3495.
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Official URL: http://dx.doi.org/10.1016/j.bpj.2014.03.047
Abstract
The established correlation between neurodegenerative disorders and intracerebral deposition of polyglutamine aggregates motivates attempts to better understand their fibrillar structure. We designed polyglutamines with a few lysines inserted to overcome the hindrance of extreme insolubility and two D-lysines to limit the lengths of β-strands. One is 33 amino acids long (PolyQKd-33) and the other has one fewer glutamine (PolyQKd-32). Both form well-dispersed fibrils suitable for analysis by electron microscopy. Electron diffraction confirmed cross-β structures in both fibrils. Remarkably, the deletion of just one glutamine residue from the middle of the peptide leads to substantially different amyloid structures. PolyQKd-32 fibrils are consistently 10–20% wider than PolyQKd-33, as measured by negative staining, cryo-electron microscopy, and scanning transmission electron microscopy. Scanning transmission electron microscopy analysis revealed that the PolyQKd-32 fibrils have 50% higher mass-per-length than PolyQKd-33. This distinction can be explained by a superpleated β-structure model for PolyQKd-33 and a model with two β-solenoid protofibrils for PolyQKd-32. These data provide evidence for β-arch-containing structures in polyglutamine fibrils and open future possibilities for structure-based drug design.
Item Type: | Journal Article | ||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Physics | ||||
Journal or Publication Title: | Biophysical Journal | ||||
Publisher: | Biophysical Society | ||||
ISSN: | 0006-3495 | ||||
Official Date: | 20 May 2014 | ||||
Dates: |
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Volume: | 106 | ||||
Number: | 10 | ||||
Page Range: | pp. 2134-2142 | ||||
DOI: | 10.1016/j.bpj.2014.03.047 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access |
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