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Stage-specific regulation of programmed cell death during oogenesis of the medfly Ceratitis capitata (Diptera, Tephritidae)
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Velentzas, Athanassios D., Nezis, I. P., Stravopodis, Dimitrios J., Papassideri, Issidora S. and Margaritis, Lukas H. (2007) Stage-specific regulation of programmed cell death during oogenesis of the medfly Ceratitis capitata (Diptera, Tephritidae). The International Journal of Developmental Biology, Volume 51 (Number 1). pp. 57-66. doi:10.1387/ijdb.062164av ISSN 0214-6282.
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Official URL: http://dx.doi.org/10.1387/ijdb.062164av
Abstract
In the present study, we describe novel features of programmed cell death in developing egg chambers occurring during mid- and late-oogenesis of the medfly Ceratitis capitata. During mid-oogenesis, the spontaneously degenerated egg chambers exhibit typical characteristics of apoptotic cell death. Their nurse cells contain fragmented DNA and fragmented actin, as revealed by TUNEL assay and immunolabelling, respectively. In vitro caspase activity assays and immunostaining procedures demonstrated that the atretic egg chambers acquired high levels of caspase activity. Distinct features of autophagic cell death were also observed during C. capitata mid-oogenesis, as revealed by the monodansylcadaverine staining approach and ultrastructural examination performed by transmission electron microscopy. Additionally, atretic egg chambers exhibit an upregulation of lysosomal proteases, as demonstrated by a procathepsin L immunolabelling procedure. At the late stages of C. capitata oogenesis, apoptosis and autophagy coexist, manifesting cell death features that are similar to the ones mentioned above, being also chaperoned by the involvement of an altered cytochrome c conformational display. We propose that apoptosis and autophagy operate synergistically during C. capitata oogenesis for a more efficient elimination of the degenerated nurse cells and abnormal egg chambers.
Item Type: | Journal Article | ||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
Journal or Publication Title: | The International Journal of Developmental Biology | ||||
Publisher: | Universidad del Pais Vasco * Servicio Editorial | ||||
ISSN: | 0214-6282 | ||||
Official Date: | 2007 | ||||
Dates: |
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Volume: | Volume 51 | ||||
Number: | Number 1 | ||||
Page Range: | pp. 57-66 | ||||
DOI: | 10.1387/ijdb.062164av | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access |
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