The Library
Identification and characterization of trimethylamine N-oxide (TMAO) demethylase and TMAO permease in Methylocella silvestris BL2
Tools
Zhu, Yijun , Jameson, Eleanor, Parslow, Rosemary A., Lidbury, Ian, Fu, Tiantian, Dafforn, Tim, Schäfer, Hendrik and Chen, Yin (2014) Identification and characterization of trimethylamine N-oxide (TMAO) demethylase and TMAO permease in Methylocella silvestris BL2. Environmental Microbiology, 16 (10). pp. 3318-3330. doi:10.1111/1462-2920.12585 ISSN 1462-2912.
|
PDF
WRAP_0676431-lf-120814-manuscript_rv3.pdf - Accepted Version - Requires a PDF viewer. Download (1422Kb) | Preview |
Official URL: http://dx.doi.org/10.1111/1462-2920.12585
Abstract
Methylocella silvestris, an alphaproteobacterium isolated from a forest soil, can grow on trimethylamine N-oxide (TMAO) as a sole nitrogen source, however, the molecular and biochemical mechanisms underpinning its growth remain unknown. Marker-exchange mutagenesisenabled the identification of several genes involved in TMAO metabolism, including Msil_3606, a permease of the amino acids-polyamine (APC) superfamily, and Msil_3603, consisting of anN-terminal domain of unknown function (DUF1989) and a C-terminal tetrahydrofolate-binding domain. Null mutants of Msil_3603 and Msil_3606 can no longer grow on TMAO. Purified Msil_3603 from recombinant Escherichia coli can convert TMAO to dimethylamine and formaldehyde (1 TMAO [RIGHTWARDS ARROW] 1 dimethylamine + 1 formaldehyde), confirming that it encodes a bona fide TMAO demethylase (Tdm). Tdm of M. silvestrisand eukaryotic TMAO demethylases have no sequence homology and contrasting characteristics. Recombinant Tdm of M. silvestris appears to be hexameric, has a high affinity for TMAO (Km= 3.3 mM; Vmax=21.7 nmolmin-1mg-1) and only catalyses demethylation of TMAO and a structural homologue, dimethyldodecylamine N-oxide. Our study has contributed to the understanding of the genetic and biochemical mechanisms for TMAO degradation in M. silvestris.
Item Type: | Journal Article | ||||||||
---|---|---|---|---|---|---|---|---|---|
Subjects: | Q Science > QR Microbiology | ||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||
Library of Congress Subject Headings (LCSH): | Gram-negative bacteria -- Genetics, Soil microbiology | ||||||||
Journal or Publication Title: | Environmental Microbiology | ||||||||
Publisher: | Blackwell | ||||||||
ISSN: | 1462-2912 | ||||||||
Official Date: | October 2014 | ||||||||
Dates: |
|
||||||||
Volume: | 16 | ||||||||
Number: | 10 | ||||||||
Page Range: | pp. 3318-3330 | ||||||||
DOI: | 10.1111/1462-2920.12585 | ||||||||
Status: | Peer Reviewed | ||||||||
Publication Status: | Published | ||||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||||
Date of first compliant deposit: | 27 December 2015 | ||||||||
Date of first compliant Open Access: | 27 December 2015 | ||||||||
Funder: | Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), University of Warwick, Natural Environment Research Council (Great Britain) (NERC) |
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |
Downloads
Downloads per month over past year