Skip to content Skip to navigation
University of Warwick
  • Study
  • |
  • Research
  • |
  • Business
  • |
  • Alumni
  • |
  • News
  • |
  • About

University of Warwick
Publications service & WRAP

Highlight your research

  • WRAP
    • Home
    • Search WRAP
    • Browse by Warwick Author
    • Browse WRAP by Year
    • Browse WRAP by Subject
    • Browse WRAP by Department
    • Browse WRAP by Funder
    • Browse Theses by Department
  • Publications Service
    • Home
    • Search Publications Service
    • Browse by Warwick Author
    • Browse Publications service by Year
    • Browse Publications service by Subject
    • Browse Publications service by Department
    • Browse Publications service by Funder
  • Help & Advice
University of Warwick

The Library

  • Login
  • Admin

Bacterial metabolism of methylated amines and identification of novel methylotrophs in Movile Cave

Tools
- Tools
+ Tools

Wischer, Daniela, Kumaresan, Deepak, Johnston, Antonia, El Khawand, Myriam, Stephenson, Jason, Hillebrand-Voiculescu, Alexandra M., Chen, Yin and Murrell, J. C. (J. Colin) (2015) Bacterial metabolism of methylated amines and identification of novel methylotrophs in Movile Cave. The ISME Journal, 9 . pp. 195-206. doi:10.1038/ismej.2014.102

[img]
Preview
PDF
WRAP_0676431-lf-130814-wischer_et_al_2014.pdf - Accepted Version - Requires a PDF viewer.

Download (913Kb) | Preview
Official URL: http://dx.doi.org/10.1038/ismej.2014.102

Request Changes to record.

Abstract

Movile Cave, Romania, is an unusual underground ecosystem that has been sealed off from the outside world for several million years and is sustained by non-phototrophic carbon fixation. Methane and sulfur-oxidising bacteria are the main primary producers, supporting a complex food web that includes bacteria, fungi and cave-adapted invertebrates. A range of methylotrophic bacteria in Movile Cave grow on one-carbon compounds including methylated amines, which are produced via decomposition of organic-rich microbial mats. The role of methylated amines as a carbon and nitrogen source for bacteria in Movile Cave was investigated using a combination of cultivation studies and DNA stable isotope probing (DNA-SIP) using 13C-monomethylamine (MMA). Two newly developed primer sets targeting the gene for gamma-glutamylmethylamide synthetase (gmaS), the first enzyme of the recently-discovered indirect MMA-oxidation pathway, were applied in functional gene probing. SIP experiments revealed that the obligate methylotroph Methylotenera mobilis is one of the dominant MMA utilisers in the cave. DNA-SIP experiments also showed that a new facultative methylotroph isolated in this study, Catellibacterium sp. LW-1 is probably one of the most active MMA utilisers in Movile Cave. Methylated amines were also used as a nitrogen source by a wide range of non-methylotrophic bacteria in Movile Cave. PCR-based screening of bacterial isolates suggested that the indirect MMA-oxidation pathway involving GMA and N-methylglutamate is widespread among both methylotrophic and non-methylotrophic MMA utilisers from the cave.

Item Type: Journal Article
Subjects: Q Science > QR Microbiology
Divisions: Faculty of Science > Life Sciences (2010- )
Library of Congress Subject Headings (LCSH): Methylotrophic bacteria, Microbial metabolism
Journal or Publication Title: The ISME Journal
Publisher: Nature Publishing Group
ISSN: 1751-7362
Official Date: January 2015
Dates:
DateEvent
January 2015Published
22 July 2014Available
22 March 2014Accepted
11 March 2014Submitted
Volume: 9
Page Range: pp. 195-206
DOI: 10.1038/ismej.2014.102
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Restricted or Subscription Access
Funder: Natural Environment Research Council (Great Britain) (NERC), University of Warwick, University of East Anglia
Grant number: NE/G017956 (NERC), NE/H016236 (NERC)

Request changes or add full text files to a record

Repository staff actions (login required)

View Item View Item

Downloads

Downloads per month over past year

View more statistics

twitter

Email us: wrap@warwick.ac.uk
Contact Details
About Us