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Bacterial metabolism of methylated amines and identification of novel methylotrophs in Movile Cave
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Wischer, Daniela, Kumaresan, Deepak, Johnston, Antonia, El Khawand, Myriam, Stephenson, Jason, Hillebrand-Voiculescu, Alexandra M., Chen, Yin and Murrell, J. C. (J. Colin) (2015) Bacterial metabolism of methylated amines and identification of novel methylotrophs in Movile Cave. The ISME Journal, 9 . pp. 195-206. doi:10.1038/ismej.2014.102 ISSN 1751-7362.
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WRAP_0676431-lf-130814-wischer_et_al_2014.pdf - Accepted Version - Requires a PDF viewer. Download (913Kb) | Preview |
Official URL: http://dx.doi.org/10.1038/ismej.2014.102
Abstract
Movile Cave, Romania, is an unusual underground ecosystem that has been sealed off from the outside world for several million years and is sustained by non-phototrophic carbon fixation. Methane and sulfur-oxidising bacteria are the main primary producers, supporting a complex food web that includes bacteria, fungi and cave-adapted invertebrates. A range of methylotrophic bacteria in Movile Cave grow on one-carbon compounds including methylated amines, which are produced via decomposition of organic-rich microbial mats. The role of methylated amines as a carbon and nitrogen source for bacteria in Movile Cave was investigated using a combination of cultivation studies and DNA stable isotope probing (DNA-SIP) using 13C-monomethylamine (MMA). Two newly developed primer sets targeting the gene for gamma-glutamylmethylamide synthetase (gmaS), the first enzyme of the recently-discovered indirect MMA-oxidation pathway, were applied in functional gene probing. SIP experiments revealed that the obligate methylotroph Methylotenera mobilis is one of the dominant MMA utilisers in the cave. DNA-SIP experiments also showed that a new facultative methylotroph isolated in this study, Catellibacterium sp. LW-1 is probably one of the most active MMA utilisers in Movile Cave. Methylated amines were also used as a nitrogen source by a wide range of non-methylotrophic bacteria in Movile Cave. PCR-based screening of bacterial isolates suggested that the indirect MMA-oxidation pathway involving GMA and N-methylglutamate is widespread among both methylotrophic and non-methylotrophic MMA utilisers from the cave.
| Item Type: | Journal Article | ||||||||||
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| Subjects: | Q Science > QR Microbiology | ||||||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||||
| Library of Congress Subject Headings (LCSH): | Methylotrophic bacteria, Microbial metabolism | ||||||||||
| Journal or Publication Title: | The ISME Journal | ||||||||||
| Publisher: | Nature Publishing Group | ||||||||||
| ISSN: | 1751-7362 | ||||||||||
| Official Date: | January 2015 | ||||||||||
| Dates: |
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| Volume: | 9 | ||||||||||
| Page Range: | pp. 195-206 | ||||||||||
| DOI: | 10.1038/ismej.2014.102 | ||||||||||
| Status: | Peer Reviewed | ||||||||||
| Publication Status: | Published | ||||||||||
| Access rights to Published version: | Restricted or Subscription Access | ||||||||||
| Date of first compliant deposit: | 27 December 2015 | ||||||||||
| Date of first compliant Open Access: | 27 December 2015 | ||||||||||
| Funder: | Natural Environment Research Council (Great Britain) (NERC), University of Warwick, University of East Anglia | ||||||||||
| Grant number: | NE/G017956 (NERC), NE/H016236 (NERC) |
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