The Library
Defective RNA processing enhances RNA silencing and influences flowering of Arabidopsis
Tools
Tools
Tools
Herr, A. J., Molnar, A., Jones, Alexandra M. and Baulcombe, D. C. (2006) Defective RNA processing enhances RNA silencing and influences flowering of Arabidopsis. Proceedings of the National Academy of Sciences of the United States of America, Volume 103 (Number 41). pp. 14994-15001. doi:10.1073/pnas.0606536103 ISSN 0027-8424.
Research output not available from this repository.
Request-a-Copy directly from author or use local Library Get it For Me service.
Official URL: http://dx.doi.org/10.1073/pnas.0606536103
Abstract
Many eukaryotic cells use RNA-directed silencing mechanisms to protect against viruses and transposons and to suppress endogenous gene expression at the posttranscriptional level. RNA silencing also is implicated in epigenetic mechanisms affecting chromosome structure and transcriptional gene silencing. Here, we describe enhanced silencing phenotype (esp) mutants in Arabidopsis thaliana that reveal how proteins associated with RNA processing and 3′ end formation can influence RNA silencing. These proteins were a putative DEAH RNA helicase homologue of the yeast PRP2 RNA splicing cofactor and homologues of mRNA 3′ end formation proteins CstF64, symplekin/PTA1, and CPSF100. The last two proteins physically associated with the flowering time regulator FY in the 3′ end formation complex AtCPSF. The phenotypes of the 3′ end formation esp mutants include impaired termination of the transgene transcripts, early flowering, and enhanced silencing of the FCA-β mRNA. Based on these findings, we propose that the ESP-containing 3′ end formation complexes prevent transgene and endogenous mRNAs from entering RNA-silencing pathways. According to this proposal, in the absence of these ESP proteins, these RNAs have aberrant 3′ termini. The aberrant RNAs would enter the RNA silencing pathways because they are converted into dsRNA by RNA-dependent RNA polymerases.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
| Journal or Publication Title: | Proceedings of the National Academy of Sciences of the United States of America | ||||
| Publisher: | National Academy of Sciences | ||||
| ISSN: | 0027-8424 | ||||
| Official Date: | 10 October 2006 | ||||
| Dates: |
|
||||
| Volume: | Volume 103 | ||||
| Number: | Number 41 | ||||
| Page Range: | pp. 14994-15001 | ||||
| DOI: | 10.1073/pnas.0606536103 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access |
Request changes or add full text files to a record
Repository staff actions (login required)
 |
View Item |
