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Analysis of the defence phosphoproteome of Arabidopsis thaliana using differential mass tagging
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Jones, Alexandra M., Bennett, Mark H., Mansfield, John W. and Grant, Murray (2006) Analysis of the defence phosphoproteome of Arabidopsis thaliana using differential mass tagging. Proteomics, Volume 6 (Number 14). pp. 4155-4165. doi:10.1002/pmic.200500172 ISSN 1615-9853.
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Official URL: http://dx.doi.org/10.1002/pmic.200500172
Abstract
Despite recent advances in proteomic technologies, quantitative analysis of the proteome remains a challenging task. Phosphorylation of proteins is central to signal transduction pathways and plays an important role in plant defence against pathogens, although the immediate targets of kinases remain elusive. Determining changes in the phosphoproteome during the defence response is a major goal in molecular plant pathology. In this first description of the novel mass tagging strategy (iTRAQ™ Applied Biosystems) applied to plant pathogen interactions, we describe early changes to the phosphoproteome of Arabidopsis thaliana during the defence response to Pseudomonas syringae pv. tomato DC3000. We identified five proteins which showed reproducible differences between a control and three different bacterial challenges, thus identifying proteins potentially phosphorylated as part of a plant basal defence response. Four of the five proteins a dehydrin, a putative p23 co-chaperone, heat shock protein 81 and a plastid-associated protein (PAP)/fibrillin, are known to be phosphorylated or have potential phosphorylation sites. One further protein, the large subunit of Rubisco, showed a significant difference between tissue undergoing the hypersensitive response and a basal defence response. We document the reproducibility, utility and problems associated with this approach.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
| Journal or Publication Title: | Proteomics | ||||
| Publisher: | Wiley-Blackwell Publishing Ltd. | ||||
| ISSN: | 1615-9853 | ||||
| Official Date: | July 2006 | ||||
| Dates: |
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| Volume: | Volume 6 | ||||
| Number: | Number 14 | ||||
| Page Range: | pp. 4155-4165 | ||||
| DOI: | 10.1002/pmic.200500172 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access |
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