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S-nitrosylation of peroxiredoxin II E promotes peroxynitrite-mediated tyrosine nitration
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Romero-Puertas, M. C., Laxa, M., Matte, A., Zaninotto, F., Finkemeier, I., Jones, Alexandra M., Perazzolli, M., Vandelle, E., Dietz, K.-J. and Delledonne, M. (2007) S-nitrosylation of peroxiredoxin II E promotes peroxynitrite-mediated tyrosine nitration. The Plant Cell, Volume 19 (Number 12). pp. 4120-4130. doi:10.1105/tpc.107.055061 ISSN 1040-4651.
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Official URL: http://dx.doi.org/10.1105/tpc.107.055061
Abstract
Nitric oxide (NO) is a free radical product of cell metabolism that plays diverse and important roles in the regulation of cellular function. S-Nitrosylation is emerging as a specific and fundamental posttranslational protein modification for the transduction of NO bioactivity, but very little is known about its physiological functions in plants. We investigated the molecular mechanism for S-nitrosylation of peroxiredoxin II E (PrxII E) from Arabidopsis thaliana and found that this posttranslational modification inhibits the hydroperoxide-reducing peroxidase activity of PrxII E, thus revealing a novel regulatory mechanism for peroxiredoxins. Furthermore, we obtained biochemical and genetic evidence that PrxII E functions in detoxifying peroxynitrite (ONOO−), a potent oxidizing and nitrating species formed in a diffusion-limited reaction between NO and O2− that can interfere with Tyr kinase signaling through the nitration of Tyr residues. S-Nitrosylation also inhibits the ONOO− detoxification activity of PrxII E, causing a dramatic increase of ONOO−-dependent nitrotyrosine residue formation. The same increase was observed in a prxII E mutant line after exposure to ONOO−, indicating that the PrxII E modulation of ONOO− bioactivity is biologically relevant. We conclude that NO regulates the effects of its own radicals through the S-nitrosylation of crucial components of the antioxidant defense system that function as common triggers for reactive oxygen species– and NO-mediated signaling events.
Item Type: | Journal Article | ||||||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||
Journal or Publication Title: | The Plant Cell | ||||||||
Publisher: | American Society of Plant Biologists | ||||||||
ISSN: | 1040-4651 | ||||||||
Official Date: | 28 December 2007 | ||||||||
Dates: |
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Volume: | Volume 19 | ||||||||
Number: | Number 12 | ||||||||
Page Range: | pp. 4120-4130 | ||||||||
DOI: | 10.1105/tpc.107.055061 | ||||||||
Status: | Peer Reviewed | ||||||||
Publication Status: | Published | ||||||||
Access rights to Published version: | Open Access (Creative Commons) |
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