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Phosphoproteomics using iTRAQ
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Jones, Alexandra M. and Nühse, Thomas S. (2011) Phosphoproteomics using iTRAQ. Methods in Molecular Biology, Volume 779 . pp. 287-302. doi:10.1007/978-1-61779-264-9_17 ISSN 1064-3745.
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Official URL: http://dx.doi.org/10.1007/978-1-61779-264-9_17
Abstract
The identification of phosphorylation on proteins has become practicable for many laboratories in recent years, largely due to improvements in mass spectrometry (MS) and the development of methods to selectively enrich for phosphorylated peptides and proteins. However, phosphorylation is a dynamic and reversible modification which plays a central role in many biological processes including intracellular signalling. Therefore, the quantitative analysis of phosphorylated proteins and peptides is a subject of intense interest. We discuss three applications of isobaric tags for relative and absolute quantitation (iTRAQ) to the analysis of phosphopeptides from a variety of sample materials.
Item Type: | Journal Article | ||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
Journal or Publication Title: | Methods in Molecular Biology | ||||
Publisher: | Springer | ||||
ISSN: | 1064-3745 | ||||
Book Title: | Plant Kinases | ||||
Official Date: | 14 July 2011 | ||||
Dates: |
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Volume: | Volume 779 | ||||
Page Range: | pp. 287-302 | ||||
DOI: | 10.1007/978-1-61779-264-9_17 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access |
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