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Macroarray expression analysis of barley susceptibility and nonhost resistance to Blumeria graminis

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Eichmann, Ruth, Biemelt, Sophia, Schäfer, Patrick, Scholz, Uwe, Jansen, Carin, Felk, Angelika, Schäfer, Wilhelm, Langen, Gregor, Sonnewald, Uwe, Kogel, Karl-Heinz and Hückelhoven, Ralph (2006) Macroarray expression analysis of barley susceptibility and nonhost resistance to Blumeria graminis. Journal of Plant Physiology, Volume163 (Number 6). pp. 657-670. doi:10.1016/j.jplph.2005.06.019

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Official URL: http://dx.doi.org/10.1016/j.jplph.2005.06.019

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Abstract

Different formae speciales of the grass powdery mildew fungus Blumeria graminis undergo basic-compatible or basic-incompatible (nonhost) interactions with barley. Background resistance in compatible interactions and nonhost resistance require common genetic and mechanistic elements of plant defense. To build resources for differential screening for genes that potentially distinguish a compatible from an incompatible interaction on the level of differential gene expression of the plant, we constructed eight dedicated cDNA libraries, established 13.000 expressed sequence tag (EST) sequences and designed DNA macroarrays. Using macroarrays based on cDNAs derived from epidermal peels of plants pretreated with the chemical resistance activating compound acibenzolar-S-methyl, we compared the expression of barley gene transcripts in the early host interaction with B. graminis f.sp. hordei or the nonhost pathogen B. graminis f.sp. tritici, respectively. We identified 102 spots corresponding to 94 genes on the macroarray that gave significant B. graminis-responsive signals at 12 and/or 24 h after inoculation. In independent expression analyses, we confirmed the macroarray results for 11 selected genes. Although the majority of genes showed a similar expression profile in compatible versus incompatible interactions, about 30 of the 94 genes were expressed on slightly different levels in compatible versus incompatible interactions.

Item Type: Journal Article
Divisions: Faculty of Science > Life Sciences (2010- )
Journal or Publication Title: Journal of Plant Physiology
Publisher: Elsevier Science BV
ISSN: 0176-1617
Official Date: 2006
Dates:
DateEvent
2006UNSPECIFIED
Volume: Volume163
Number: Number 6
Page Range: pp. 657-670
DOI: 10.1016/j.jplph.2005.06.019
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Restricted or Subscription Access

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