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Identification of serine 348 on the apelin receptor as a novel regulatory phosphorylation site in apelin-13-induced G protein-independent biased signaling
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Chen, X., Bai, B., Tian, Y., Du, H. and Chen, Jing (2014) Identification of serine 348 on the apelin receptor as a novel regulatory phosphorylation site in apelin-13-induced G protein-independent biased signaling. Journal of Biological Chemistry . doi:10.1074/jbc.M114.574020 ISSN 0021-9258.
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Official URL: http://dx.doi.org/10.1074/jbc.M114.574020
Abstract
Phosphorylation plays vital roles in the regulation of GPCRs functions. The apelin and apelin receptor (APJ) system is involved in the regulation of cardiovascular function and central control of body homeostasis. Here, using tandem mass spectrometry, we first identified phosphorylated serine residues in the C-terminus of APJ. To determine the role of phosphorylation sites in APJ mediated G protein-dependent and -independent signaling and function, we induced a mutation in the C-terminal serine residues and examined their effects on the interaction between APJ with G protein or GRK/β-arrestin and their down-streaming signaling. Mutation of serine 348 led to an elimination of both GRK and β-arrestin recruitment to APJ induced by apelin-13. Moreover, APJ internalization and G protein-independent ERK signaling were also abolished by point mutation at serine 348. In contrast, this mutant at serine residues had no demonstrable impact on apelin-13 induced G protein activation and its intracellular signaling. These findings suggest that mutation of serine 348 resulted in GRK/β-arrestin inactive. However there was no change in the active G protein thus APJ conformation was biased. These results provide important information on the molecular interplay and impact of the APJ function, which may be extrapolated to design novel drugs for cardiac hypertrophy based on this biased signal pathway.
| Item Type: | Journal Article | ||||||||
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| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Translational & Experimental Medicine > Metabolic and Vascular Health (- until July 2016) Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Translational & Experimental Medicine Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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| Journal or Publication Title: | Journal of Biological Chemistry | ||||||||
| Publisher: | American Society for Biochemistry and Molecular Biology | ||||||||
| ISSN: | 0021-9258 | ||||||||
| Official Date: | 30 September 2014 | ||||||||
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| DOI: | 10.1074/jbc.M114.574020 | ||||||||
| Status: | Peer Reviewed | ||||||||
| Publication Status: | Published | ||||||||
| Access rights to Published version: | Restricted or Subscription Access |
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