Skip to content Skip to navigation
University of Warwick
  • Study
  • |
  • Research
  • |
  • Business
  • |
  • Alumni
  • |
  • News
  • |
  • About

University of Warwick
Publications service & WRAP

Highlight your research

  • WRAP
    • Home
    • Search WRAP
    • Browse by Warwick Author
    • Browse WRAP by Year
    • Browse WRAP by Subject
    • Browse WRAP by Department
    • Browse WRAP by Funder
    • Browse Theses by Department
  • Publications Service
    • Home
    • Search Publications Service
    • Browse by Warwick Author
    • Browse Publications service by Year
    • Browse Publications service by Subject
    • Browse Publications service by Department
    • Browse Publications service by Funder
  • Help & Advice
University of Warwick

The Library

  • Login
  • Admin

Exploring the functional soil-microbe interface and exoenzymes through soil metaexoproteomics

Tools
- Tools
+ Tools

Johnson-Rollings, Ashley S., Wright, H., Masciandaro, Grazia, Macci, Cristina, Doni, Serena, Calvo-Bado, Leo A., Slade, Susan E., Plou, Carlos Vallin and Wellington, E. M. H. (2014) Exploring the functional soil-microbe interface and exoenzymes through soil metaexoproteomics. The ISME Journal, Volume 8 (Number 10). pp. 2148-2150. doi:10.1038/ismej.2014.130

[img]
Preview
PDF
WRAP_Wellington_ismej2014130a.pdf - Published Version - Requires a PDF viewer.
Available under License Creative Commons Attribution Non-commercial Share Alike.

Download (563Kb) | Preview
Official URL: http://dx.doi.org/10.1038/ismej.2014.130

Request Changes to record.

Abstract

Functionally important proteins at the interface of cell and soil are of potentially low abundance when compared with commonly recovered intracellular proteins. A novel approach was developed and used to extract the metaexoproteome, the subset of proteins found outside the cell, in the context of a soil enriched with the nitrogen-containing recalcitrant polymer chitin. The majority of proteins recovered was of bacterial origin and localized to the outer membrane or extracellular milieu. A wide variety of transporter proteins were identified, particularly those associated with amino-acid and phosphate uptake. The metaexoproteome extract retained chitinolytic activity and we were successful in detecting Nocardiopsis-like chitinases that correlated with the glycoside hydrolase family 18 (GH18) chi gene data and metataxonomic analysis. Nocardiopsis-like chitinases appeared to be solely responsible for chitinolytic activity in soil. This is the first study to detect and sequence bacterial exoenzymes with proven activity in the soil enzyme pool.

Item Type: Journal Article
Subjects: S Agriculture > S Agriculture (General)
Divisions: Faculty of Science > Life Sciences (2010- )
Library of Congress Subject Headings (LCSH): Soil biochemistry, Extracellular enzymes
Journal or Publication Title: The ISME Journal
Publisher: Nature Publishing Group
ISSN: 1751-7362
Official Date: 8 July 2014
Dates:
DateEvent
8 July 2014Published
3 June 2014Accepted
16 May 2014Submitted
Volume: Volume 8
Number: Number 10
Number of Pages: 3
Page Range: pp. 2148-2150
DOI: 10.1038/ismej.2014.130
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Restricted or Subscription Access
Funder: Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Seventh Framework Programme (European Commission) (FP7), Natural Environment Research Council (Great Britain) (NERC)
Grant number: KBBE-222625 (FP7, EU METAEXPLORE project (KBBE-222625))

Request changes or add full text files to a record

Repository staff actions (login required)

View Item View Item

Downloads

Downloads per month over past year

View more statistics

twitter

Email us: wrap@warwick.ac.uk
Contact Details
About Us