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Improving the isolation of anaerobes on solid media: the example of the fastidious Methanosaeta

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Carbonero, Franck, Oakley, Brian B. and Purdy, Kevin J.. (2010) Improving the isolation of anaerobes on solid media: the example of the fastidious Methanosaeta. Journal of Microbiological Methods, Vol.80 (No.2). pp. 203-205. ISSN 0167-7012

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Official URL: http://dx.doi.org/10.1016/j.mimet.2009.11.013

Abstract

Methanosaeta spp. are globally important biogenic methane producers with only three strains described due to isolation difficulties. Here, clonal axenic isolates from estuarine sediments were obtained using alternative gelling and reducing agents. It is suggested that more systematic approaches with various combinations of media components will help to cultivate difficult-to-isolate anaerobes. (C) 2009 Elsevier B.V. All rights reserved.

Item Type: Journal Article
Subjects: Q Science > QD Chemistry
Q Science > QR Microbiology
Divisions: Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010)
Journal or Publication Title: Journal of Microbiological Methods
Publisher: Elsevier
ISSN: 0167-7012
Date: February 2010
Volume: Vol.80
Number: No.2
Number of Pages: 3
Page Range: pp. 203-205
Identification Number: 10.1016/j.mimet.2009.11.013
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Restricted or Subscription Access
Funder: EU Commission
Grant number: MEXT-CT-2005-024112
URI: http://wrap.warwick.ac.uk/id/eprint/6340

Data sourced from Thomson Reuters' Web of Knowledge

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