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Eukarya 18S rRNA gene diversity in the sea surface microlayer: implications for the structure of the neustonic microbial loop
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Cunliffe, Michael and Murrell, J. C. (J. Colin) (2010) Eukarya 18S rRNA gene diversity in the sea surface microlayer: implications for the structure of the neustonic microbial loop. ISME Journal, Vol.4 (No.3). pp. 455-458. ISSN 1751-7362
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Official URL: http://dx.doi.org/10.1038/ismej.2009.133
Abstract
We have previously shown that there is a consistent and reproducible bacterioneuston community in the surface microlayer during a fjord mesocosm experiment. One possible cause of the surface microlayer-specific bacterial community is a surface microlayer-specific protist community selectively grazing on the bacterioneuston. We determined protist community structures using Eukarya 18S rRNA gene denaturing gradient gel electrophoresis (DGGE) and subsequent DGGE band sequencing using DNA samples that were collected from the surface microlayer and subsurface water of the mesocosms. As with bacterial communities, protist community structure was consistently different in the surface microlayer when compared with subsurface water. In particular, the protist community in the surface microlayer was dominated by Cercozoa, which were not detected in the subsurface water, and Ciliophora. The ISME Journal (2010) 4, 455-458; doi:10.1038/ismej.2009.133; published online 10 December 2009
| Item Type: | Journal Item |
|---|---|
| Subjects: | Q Science > QH Natural history > QH301 Biology Q Science > QR Microbiology |
| Divisions: | Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010) |
| Journal or Publication Title: | ISME Journal |
| Publisher: | Nature Publishing Group |
| ISSN: | 1751-7362 |
| Date: | March 2010 |
| Volume: | Vol.4 |
| Number: | No.3 |
| Number of Pages: | 4 |
| Page Range: | pp. 455-458 |
| Identification Number: | 10.1038/ismej.2009.133 |
| Status: | Peer Reviewed |
| Publication Status: | Published |
| Access rights to Published version: | Restricted or Subscription Access |
| Funder: | Natural Environment Research Council (Great Britain) (NERC), NERC-Surface Ocean Lower Atmosphere Study (SOLAS)-directed programme |
| Grant number: | NE/E011446/1 |
| URI: | http://wrap.warwick.ac.uk/id/eprint/6345 |
Data sourced from Thomson Reuters' Web of Knowledge
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