Carter, Charlotte E., Szmidt‐Jaworska, A., Hughes, M., Thomas, Brian and Jackson, Stephen D. (2000) Phytochrome regulation of phytochrome A mRNA levels in the model short-day-plant Pharbitis nil. Journal of Experimental Botany, Volume 51 (Number 345). pp. 703-711. doi:10.1093/jexbot/51.345.703

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Abstract

The exposure of dark‐grown Pharbitis nil seedlings to continuous R induces a rapid decrease in PHYA mRNA abundance with a half‐life of about 2 h. A 5 min R pulse also induces this decline, and the effect is partially reversible by subsequent FR irradiation, confirming that the regulation of expression is mediated via the Pfr form of a phytochrome. When de‐etiolated seedlings are returned to darkness after a W photoperiod, PHYA mRNA slowly reaccumulates from 20% to 50% of the dark level within 24 h. The rate of reaccumulation is greatly accelerated by the removal of Pfr with a FR pulse, resulting in reaccumulation to 100% within approximately 11 h. Without FR irradiation PHYA mRNA expression remains fully repressed for at least 11 h after the end of the photoperiod, suggesting that the controlling Pfr is highly stable.

Item Type:	Journal Article
Divisions:	Faculty of Science > Life Sciences (2010- )
Journal or Publication Title:	Journal of Experimental Botany
Publisher:	Oxford University Press
ISSN:	0022-0957
Official Date:	April 2000
Dates:	Date Event April 2000 Published
Volume:	Volume 51
Number:	Number 345
Page Range:	pp. 703-711
DOI:	10.1093/jexbot/51.345.703
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access

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