The Library
Bacterial drug tolerance under clinical conditions is governed by anaerobic adaptation but not anaerobic respiration
Tools
Hemsley, Claudia M., Luo, Jamie X., Andreae, Clio A., Butler, Clive S., Soyer, Orkun S. and Titball, Richard W. (2014) Bacterial drug tolerance under clinical conditions is governed by anaerobic adaptation but not anaerobic respiration. Antimicrobial Agents and Chemotherapy, Volume 58 (Number 10). pp. 5775-5783. doi:10.1128/AAC.02793-14 ISSN 0066-4804.
|
PDF
WRAP_Antimicrob. Agents Chemother.-2014-Hemsley-5775-83.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution. Download (1827Kb) | Preview |
Official URL: http://dx.doi.org/10.1128/AAC.02793-14
Abstract
Non-inherited antibiotic resistance is a phenomenon whereby a subpopulation of genetically identical bacteria displays phenotypic tolerance to antibiotics. We show that, compared to E. coli, the clinically relevant genus Burkholderia displays much higher levels of cells that tolerate ceftazidime. Measuring the dynamics of the formation of drug-tolerant cells, under conditions that mimic in vivo infections, we show that in Burkholderia oxygen levels affect the formation of these cells. The drug-tolerant cells are characterised by an anaerobic metabolic signature and can be eliminated by oxygenation of the system, or by the addition of nitrate. The transcriptome profile suggest that these cells are not dormant persister cells, and are likely to be drug-tolerant as a consequence of the up-regulation of anaerobic nitrate respiration, efflux pumps, beta lactamases and stress response proteins. These findings have important implications for the treatment of chronic bacterial infections and the methodologies and conditions which are used to study drug-tolerant and persister cells in vitro.
Item Type: | Journal Article | ||||||||
---|---|---|---|---|---|---|---|---|---|
Subjects: | Q Science > QR Microbiology | ||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||
Library of Congress Subject Headings (LCSH): | Drug resistance in microorganisms | ||||||||
Journal or Publication Title: | Antimicrobial Agents and Chemotherapy | ||||||||
Publisher: | American Society for Microbiology | ||||||||
ISSN: | 0066-4804 | ||||||||
Official Date: | 21 July 2014 | ||||||||
Dates: |
|
||||||||
Volume: | Volume 58 | ||||||||
Number: | Number 10 | ||||||||
Page Range: | pp. 5775-5783 | ||||||||
DOI: | 10.1128/AAC.02793-14 | ||||||||
Status: | Peer Reviewed | ||||||||
Publication Status: | Published | ||||||||
Date of first compliant deposit: | 26 May 2016 | ||||||||
Date of first compliant Open Access: | 26 May 2016 | ||||||||
Funder: | Wellcome Trust (London, England), Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC) | ||||||||
Grant number: | WT085162AIA (WT), BB/1024631/1 (BBSRC) |
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |
Downloads
Downloads per month over past year