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A growth quantification assay for hyaloperonospora arabidopsidis isolates in Arabidopsis thaliana

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Tomé, Daniel F. A., Steinbrenner, Jens and Beynon, Jim (2014) A growth quantification assay for hyaloperonospora arabidopsidis isolates in Arabidopsis thaliana. In: Plant-Pathogen Interactions. Methods in Molecular Biology, Volume 1127 . Humana Press, pp. 145-158. ISBN 9781627039857

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Official URL: http://dx.doi.org/10.1007/978-1-62703-986-4_12

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Abstract

There is a considerable interest in determining the role of individual oomycete effectors in promoting disease. Widely used strategies are based on manipulating effector-expression levels in the pathogen and by over-expressing particular effectors in the host by genetic transformation. In the case of the oomycete, Hyaloperonospora arabidopsidis (Hpa) genetic manipulation is not yet possible, so over-expression of predicted effectors in stably transformed Arabidopsis lines is used to investigate their capability for promoting virulence. Here, we describe a technique for quantifying pathogen growth based on the counting of asexual reproductive structures called sporangiophores in the compatible interaction between the Hpa isolate Noks1 and the Col-0 Arabidopsis accession.

Item Type: Book Item
Divisions: Faculty of Science > Life Sciences (2010- )
Faculty of Science > Centre for Systems Biology
Series Name: Methods in Molecular Biology
Publisher: Humana Press
ISBN: 9781627039857
ISSN: 1064-3745
Book Title: Plant-Pathogen Interactions
Official Date: 2014
Dates:
DateEvent
2014UNSPECIFIED
Volume: Volume 1127
Page Range: pp. 145-158
DOI: 10.1007/978-1-62703-986-4_12
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Restricted or Subscription Access

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