Tomé, Daniel F. A., Steinbrenner, Jens and Beynon, Jim (2014) A growth quantification assay for hyaloperonospora arabidopsidis isolates in Arabidopsis thaliana. In: Plant-Pathogen Interactions. Methods in Molecular Biology, Volume 1127 . Humana Press, pp. 145-158. ISBN 9781627039857

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Abstract

There is a considerable interest in determining the role of individual oomycete effectors in promoting disease. Widely used strategies are based on manipulating effector-expression levels in the pathogen and by over-expressing particular effectors in the host by genetic transformation. In the case of the oomycete, Hyaloperonospora arabidopsidis (Hpa) genetic manipulation is not yet possible, so over-expression of predicted effectors in stably transformed Arabidopsis lines is used to investigate their capability for promoting virulence. Here, we describe a technique for quantifying pathogen growth based on the counting of asexual reproductive structures called sporangiophores in the compatible interaction between the Hpa isolate Noks1 and the Col-0 Arabidopsis accession.

Item Type:	Book Item
Divisions:	Faculty of Science > Life Sciences (2010- ) Faculty of Science > Centre for Systems Biology
Series Name:	Methods in Molecular Biology
Publisher:	Humana Press
ISBN:	9781627039857
ISSN:	1064-3745
Book Title:	Plant-Pathogen Interactions
Official Date:	2014
Dates:	Date Event 2014 UNSPECIFIED
Volume:	Volume 1127
Page Range:	pp. 145-158
DOI:	10.1007/978-1-62703-986-4_12
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access

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